Outline . Questions from last lecture? P. 40 questions on Pax6 gene Mechanism of Transcription Activation Transcription Regulatory elements Comparison between yeast and mammals Examples of mammalian transcriptional control regions Transcription Factors (activators and repressors)
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Results in different sites of transcription initiation and
alternative splicing in different tissues
Transcript a is detected in lense
and pancreas. Lacks exons 1 and α
Fig. 7-7 Lodish et al. 2008
different transcripts in different tissues
detected in retina
lacks exons 0-4
contains exon α
exon α is unique to vertebrates
Reporter gene activity
What can you tell from comparing mutants 1, 2, 3 & 4?
Fig 7.8 – example of how a SALLI gene enhancer was identified in an intergenic region 500 kb downstream of the SALL1 gene
Purple = in text book readings
Fig. 7-22. Lodish
What are 3 different functions of proteins that regulate transcription by binding to specific DNA sequences?
General transcription factors e.g. TATA binding protein
At home: draw a picture of a eukaryotic promoter at the time of transcription initiation (include all proteins expected to be there)
Enhanceosome on the β-interferon enhancer
What determines whether a given protein is found associated with the enhancer?
COLDAIR transcription is induced by cold
COLDAIR noncoding RNA binds Polycomb group proteins
FLC locus is methylated on histone H3 Silencing
Science 331: 76-79 (2011)