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Comprehensive Biophysical Tools in Chromatography for Effective Molecule Separation

Learn about different types of chromatography techniques including partition, adsorption, TLC, and more to optimize separations based on molecule properties. Explore advanced tools, column supports, and elution strategies for efficient results.

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Comprehensive Biophysical Tools in Chromatography for Effective Molecule Separation

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  1. Chromatography Biophysical Tools '04 - Chromatography

  2. Partition chromatography • redistribution (partition) of the molecules between the stationary and mobile phase Plate model of chromatography of mixture of compounds partitioning 1:1 (bold) and 1:3 (italics) between the mobile and stationary phase. • larger number of plates – better separation Biophysical Tools '04 - Chromatography

  3. Adsorption • stationary phases: alumina, activated carbon, hydroxyapatite Column Support Biophysical Tools '04 - Chromatography

  4. TLC and GLC Thin layer chromatography Gas-liquid chromatography Biophysical Tools '04 - Chromatography

  5. Kinds of chromatography Biophysical Tools '04 - Chromatography

  6. Gel exclusion Biophysical Tools '04 - Chromatography

  7. Molecular Weight by Gel Exclusion Biophysical Tools '04 - Chromatography

  8. rate column height Optimization Biophysical Tools '04 - Chromatography

  9. Sample size Biophysical Tools '04 - Chromatography

  10. Ion-Exchange chromatography Biophysical Tools '04 - Chromatography

  11. Elution gradient salt gradients or pH Biophysical Tools '04 - Chromatography

  12. pH of the elution Biophysical Tools '04 - Chromatography

  13. HPLC • Extremely large number of plates  high pressure rate of elution Biophysical Tools '04 - Chromatography

  14. Other types of chromatography: • Affinity (couple ligand to CNBr-activated Sepharose) • Hydrophobic (low pressure RP-HPLC equivalent) Biophysical Tools '04 - Chromatography

  15. Filtration and Dialysis Dialysis change buffer often • Filtration • nitrocellulose & fiberglass • Centricon series Reverse dialysis Biophysical Tools '04 - Chromatography

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