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Comprehensive Analysis of Prostate Cancer Specimens Using Radical Prostatectomy Techniques

This detailed guide covers the transverse section of the prostate during radical prostatectomy, including sampling techniques, frozen and permanent sections, and tissue processing for diagnostic pathology. Learn about obtaining tumor and non-tumor samples, seminal vesicle sections, and tissue preservation methods.

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Comprehensive Analysis of Prostate Cancer Specimens Using Radical Prostatectomy Techniques

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  1. Prostate: Transverse section

  2. Radical Prostatectomy Bladder neck Apex

  3. Radical Prostatectomy Base/ Bladder Neck Apex Posterior quadrants of alternate slices have been removed and frozen after inking the surface

  4. Lateral aspect Schematic of how samples of tumor are obtained from a radical prostatectomy specimen, without compromising sampling or diagnostic pathology data Seminal Vesicle Permanent section Permanent section right left 1 7 2 6 3 5 4 5R 5 5R 5L 5L Fresh, non-frozen tissue for flow sorting Frozen section Unfixed tissue for RNA & DNA left right 4R Frozen section 4 4R 4L 4L Permanent section left right permanent section 3R 3 3R 5L 3L = Prostate carcinoma

  5. Human prostate (lateral view) Obtaining samples of non-tumortissue from a radical prostatectomy specimen. Seminal Vesicle Permanent section Fixed tissue: In-situ hybridization right 1 left 7 2 6 3 5 4 5R 5 5R 5L 5L Fresh, non-frozen tissue: cell sorting This block is snap frozen (isopentane in liquid N2), within < 20 minutes of devitalization Frozen tissue: Immunostains Laser capture right left 4R 4 4R 4L 4L Frozen section Permanent section left right Permanent section 3R 3 3R 5L 3L = Prostate carcinoma

  6. Prostate (transverse section)

  7. Normal prostate parenchyma (high magnification) Basal epithelial cells Secretory (luminal) epithelial cells Fibromuscular stromal cells

  8. Normal prostate parenchyma (low magnification) fibromuscular stroma normal glands lymphocytes

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