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ISH and FISH - PowerPoint PPT Presentation


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In situ Hybridization (ISH) is a method that allows to localize and detect nucleic acid sequences within structurally intact cells or morphologically preserved tissues sections. Fluorescence in situ hybridization (FISH) is a kind of ISH which uses fluorescent probes binding parts of the chromosome to show a high degree of sequence complementarity.\n

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ish and fish

ISH and FISH

In situ Hybridization (ISH) is a method that allows to localize and detect nucleic acid

sequences within structurally intact cells or morphologically preserved tissues

sections. Fluorescence in situ hybridization (FISH) is a kind of ISH which uses

fluorescent probes binding parts of the chromosome to show a high degree of

sequence complementarity. The basic principles for FISH and all other methods of in

situ hybridization are the same, except one is utilizing a fluorescence probe to detect

specific nucleotide

sequences within

immunohistochemistry which usually localize proteins in tissue sections. In situ

hybridization can be performed on a variety of targets, including RNA within cells,

DNA in metaphase chromosome preparations obtained from mitotic cells, or DNA in

interphase nuclei from cells in the non-mitotic phases of the cell cycle. In addition to

the advantages of detection in morphologic context, in situ hybridization has become

popular also because of its high sensitivity in nucleic acid detection. In situ

hybridization has been widely used for research applications, including clinical

cytogenetics, gene mapping, tumor biology and studies of chromosome evolution.

cells and

tissues. They differ

from

Choice of Probe

Probe is critical to in situ hybridization, and a right probe can help you achieve your

goals. Not only the probe types but also the label of probe should you take into

account when you choose a probe for in situ hybridization.

https://www.creative-diagnostics.com/in-situ-hybridization-and-fluorescence-in-situ-

hybridization.htm