89 - RNA polymerase 1 Subunit C

Aug 26, 2014

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90 - Putative DNA primase # GfIV-C20 ORF1. 92 - ALI-like protein. 95 - Zinc finger protein. 89 - RNA polymerase 1 Subunit C. 91 - Unknwon GfV-like protein. 93 - D5 pox-like protein # CIV 184R & GfIV-C20 ORF1. 94 - Putative chromosomal replication protein. 1. 5. 10. 13.

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89 - RNA polymerase 1 Subunit C

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90 - Putative DNA primase # GfIV-C20 ORF1 92 - ALI-like protein 95 - Zinc finger protein 89 - RNA polymerase 1 Subunit C 91 - Unknwon GfV-like protein 93 - D5 pox-like protein # CIV 184R & GfIV-C20 ORF1 94 - Putative chromosomal replication protein 1 5 10 13

Activities of RNA Polymerase

Activities of RNA Polymerase. sequence specific DNA binding -promoters melts DNA to reveal the template strand selects ribonucleotide (not deoxynucleotides) that anneals to template strand polymerizes RNA strand translocates on DNA template, during which it must:

598 views • 44 slides

Regulatory effects on RNA polymerase

Regulatory effects on RNA polymerase. Binding constants Rate constants Measuring K B. Multiple steps in initiation and elongation by RNA polymerase are targets for regulation. RNA Polymerase has to * bind to promoters, * form an open complex, * initiate transcription,

305 views • 19 slides

E. coli RNA polymerase (redux)

E. coli RNA polymerase (redux). Functions of other subunits: α - binds the UP element upstream of very strong promoters (rRNA), and some protein activators. β - active site of Pol, also binds  , nascent RNA, RNA-DNA hybrid, and DS DNA in front of bubble.

579 views • 23 slides

RNA polymerase II The central enzyme of gene expression

TF. TBP. TATA. Promoter. RNA polymerase II The central enzyme of gene expression. Enzymatic function. Enzymatic reaction: NTP  RNA + PPi (1969) RNA n + NTP + (Mg ++ + templat) = RNA n+1 + PP i Processive - can transcribe 10 6 bp template without dissociation

621 views • 48 slides

a. DNA ligase b. DNA polymerase c. RNA polymerase d. Restriction enzyme

a. DNA ligase b. DNA polymerase c. RNA polymerase d. Restriction enzyme e. Reverse transcriptase f. Transformation 1. Enzyme found in retroviruses that produce DNA from an RNA template. 2. Enzyme used during replication to attach Okazaki fragments to each other.

508 views • 16 slides

regulatory subunit

SAH. SAM. regulatory subunit. CH3. B. B. Methyltransferase. A. C. A. C. A. C. Active PP2A. Inactive PP2A. SAH. P-tau. Tau. ATP. Kinases. ADP. +. Presenilin 1.  -amyloid. Amyloid precursor protein. Secretases overexpression. +. HCY. DNA-hypomethylation. Accumulates

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PFA0505c DNA-directed RNA polymerase 2 subunit, putative

Legend for Additional files 1, 2, 3 and 4 Additional files 1, 2, 3 and 4. Sets of over-represented motifs identified in the present study, by 3 motif-discovery programs, in the upstream regions of each

471 views • 40 slides

T7 RNA Polymerase in a High Magnetic Field

T7 RNA Polymerase in a High Magnetic Field. Kim Wadelton Sweet Briar College. Sponsored by: The National High Magnetic Field Laboratory The University of Florida The National Science Foundation (NSFDMR-0305371) and NASA (NNA045561). Marianna Worczak Clarkson University James Ch. Davis

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RNA polymerase, the enzyme that transcribes the genetic code

RNA polymerase, the enzyme that transcribes the genetic code Human body: more than 200 cell types, all have same DNA Different cell types because differences in gene expression patterns in time and space.

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Molecular basis of rna -dependant rna polymerase II activity

Molecular basis of rna -dependant rna polymerase II activity. Elisabeth Lehmann et. al. Introduction. Catalyses DNA-Dependant RNA synthesis Structure has 12 subunits ~ 550 kDa Key polymerase in transcription. Introduction. Error Rate : 10 -3

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Eukaryotic RNA polymerase 18S+ 30 proteins= 40S

Initiation of rDNA transcription Two transcription factors, UBF and SL1, bind cooperatively to the rDNA promoter and recruit RNA polymerase I to form an initiation complex. One subunit of SL1 is the TATA-binding protein (TBP) and the other is TBP- associated Factor (TAF).

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89% C-Se-C

Se Ca Fe. o. o. A. D. o. o. 100% C-Se-C. o. o. 600 m m. o. E. C. B. 46% C-Se-C. 89% C-Se-C. Se Ca Zn. 23% SeO 3 2-. 31% Se 0. 11% Se 0.

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Formation of RNA Polymerase II pre-initiation complex

Formation of RNA Polymerase II pre-initiation complex. IID contains TBP that binds TATA box. IIA stabilizes IID binding to promoter. IIB binds initiation sequence. Pol II binds IIB. IIE stimulates transcription. IIH has kinase and helicase activity. RNA Synthesis in E. Coli. Transcription

1.07k views • 50 slides

E. coli RNA Polymerase

E. coli RNA Polymerase. M.Prasad Naidu MSc Medical Biochemistry, Ph.D.Research Scholar. RNA Polymerase. Catalyzes the formation of the phosphodiester bonds between the nucleotides (sugar to phosphate) Uncoils the DNA, adds the nucleotide one at a time in the 5’ to 3’ fashion

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RNA Polymerase

RNA Polymerase. mRNA being formed from a strand of DNA by proteins. HUMAN ATGCTTCCGGGCACATT...ACGAG CHIMP ATGCTTCCGGGCACATT...ACGAG RAT AT A.. TC G G A G T ACAT CGCA ACGA A HUMAN C.TCAGTGCCTGCCGGAAATCTCCC CHIMP C.TCAGT AT CTGCCGGAAATCTCCC RAT C A T TG GTGCCT TTT G C A TG T T T G CC

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C sh RNA-LPS

Figure S1. a. H sh RNA-Unt. C sh RNA-Unt. H sh RNA-LPS. C sh RNA-LPS.

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RNA Polymerase binding

RNA Polymerase binding. *Constitutive or basal level transcription. Allosteric activation of RNA polymerase. Inhibition of transcription. DNA-protein interactions. Negative Control of the lac Operon. Proposed CAP-cAMP Activation of lac Transcription. Transcription in Eukaryotes.

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D&C 89

D&C 89. The revelation known as the Word of Wisdom can be broken into four parts:. 1)Explanation for its existence (1-4) 2)Don’ts (5-9) 3)Dos (10-17) 4)Word of Wisdom Promise (18-21). The Circumstances on Feb 27, 1833.

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ECOLI RNA POLYMERASE

BIOCHEMISTRY

340 views • 17 slides

Formation of RNA Polymerase II pre-initiation complex

566 views • 50 slides

RNA polymerase II The central enzyme of gene expression

543 views • 52 slides

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89 - RNA polymerase 1 Subunit C

89 - RNA polymerase 1 Subunit C

Presentation Transcript

Activities of RNA Polymerase

Regulatory effects on RNA polymerase

E. coli RNA polymerase (redux)

RNA polymerase II The central enzyme of gene expression

a. DNA ligase b. DNA polymerase c. RNA polymerase d. Restriction enzyme

regulatory subunit

PFA0505c DNA-directed RNA polymerase 2 subunit, putative

T7 RNA Polymerase in a High Magnetic Field

RNA polymerase, the enzyme that transcribes the genetic code

Molecular basis of rna -dependant rna polymerase II activity

Eukaryotic RNA polymerase 18S+ 30 proteins= 40S

89% C-Se-C

Formation of RNA Polymerase II pre-initiation complex

E. coli RNA Polymerase

RNA Polymerase

C sh RNA-LPS

RNA Polymerase binding

D&amp;C 89

ECOLI RNA POLYMERASE

Formation of RNA Polymerase II pre-initiation complex

RNA polymerase II The central enzyme of gene expression

D&C 89