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200. 200. 160. 160. 120. 120. 80. 80. 40. 40. 0. 0. 10 0. 10 0. 10 1. 10 1. 10 2. 10 2. 10 3. 10 3. 10 4. 10 4. γδ T cells. A. B. αβ T cells. Counts. CD25. Supplemental figure 1

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200

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γδ T cells

A

B

αβ T cells

Counts

CD25

Supplemental figure 1

Distinct stimulation status of αβ and γδ T cells induced by CD28 and cross-bound CD3 mAbs. Magnetically isolated αβ (A) and γδ (B) T cells were cultured in the abscence (black line) or presence (grey line) of CD28 and 10 µg/ml cross-linked plate-bound anti-CD3 mAb for 72h. Activation status was determined by CD25 expression measured by flow cytometry as described in Materials and Methods.


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+ anti-TLR-2

+ anti-TLR-4

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B

Counts

FasL

Supplemental figure 2

E. coli Nissle 1917 did not increase FasL expression on stimulated γδ T cells cultured with anti-TLR-2 and anti-TLR-4 mAbs. Magnetically isolated γδ T cells were stimulated with IPP and IL-2 and cultured with blocking anti-TLR-2 (A) or anti-TLR-4 (B) mAbs. γδ T cells were incubated in the absence (grey line) or presence (black line) of 25% (vol/vol) E. coli Nissle 1917-CM. FasL expression was determined by flow cytometry as described in Material and Methods. The graphs represent typical donors and are representative of three individual experiments.


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