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Gram. ’. s. Gram’s. (Primary stain). (Primary stain). Crystal . Alcohol . Safranin. Gram’s . Gram. ’. s . iodine. violet. Crystal . 1. 2. 4. 5. 3. Answers: 1. Gram-negative spirilli 2. Gram-positive bacilli 3. Gram-positive cocci

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Presentation Transcript
slide2

Gram

s

Gram’s

(Primary stain)

(Primary stain)

Crystal

Alcohol

Safranin

Gram’s

Gram

s

iodine

violet

Crystal

slide4

1

2

4

5

3

Answers: 1. Gram-negative spirilli 2. Gram-positive bacilli 3. Gram-positive cocci

4. Gram-negative bacilli 5. Gram-positive cocci

slide5

Culture A should be composed of these two bacteria

Micrococcus lutea (Gram-positive sarcinae cocci in cuboidal packets of four to eight, stained purple-violet) and Klebsiella pneumoniae (very small Gram-negative bacilli stained red-pink).

Micrococcuslutea produces tiny bright yellow colonies and Klebsiellapneumoniae produces larger, mucoid white colonies.

slide6

Culture B should be composed of these two bacteria

Staphylococcus aureus (irregular clusters of small Gram-positive cocci stained purple-violet) and Serratiamarcesens (very small Gram-negative bacilli stained red-pink)

Staphylococcusaureus produces small pale golden colonies and Serratia marcesens produces larger reddishcolonies.

slide7

Bacillus megaterium (large, thick Gram-positive bacilli, often with endospores, stained purple-violet) and Escherichia coli (small Gram-negative bacilli stained red-pink).

T

O

Escherichia coli produces small irregular translucent (T) white colonies and Bacillus megaterium produces larger, more rounded opaque (O) white colonies. These are sometimes difficult to distinguish in room light.

Culture C should be composed of these two bacteria

slide8

These photos show a Gram stain of a bacterial infection (x1000).

Distinguish the bacteria and the human cells in each smear.

Which photo (top or bottom) shows Gram-negative bacteria? Are they bacilli or cocci?

1

2

Answers:

1 and 4: Human cells

2 and 3: Bacteria

Gram-negative cocci in bottom photo

4

3