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Amino Acid Enzymes. Amino Acid Enzymes. Enzymes that attack Amino Acids 1. Cysteine desulfhydrase 2. Lysine decarboxylase 3. Phenylalanine deaminase. Amino Acid Structure. Amino Acid Enzymes. Cysteine desulfhydrase

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Presentation Transcript
amino acid enzymes1
Amino Acid Enzymes
  • Enzymes that attack Amino Acids

1. Cysteine desulfhydrase

2. Lysine decarboxylase

3. Phenylalanine deaminase

amino acid enzymes2
Amino Acid Enzymes

Cysteine desulfhydrase

Cysteine Hydrogen Sulfide + ammonia + Pyruvic acid

peptone iron agar deeps
Peptone Iron Agar Deeps
  • Peptone – protein rich in cysteine
  • Iron - Fe
slide6

Cysteinedesulfhydrase

Cysteine Hydrogen Sulfide + ammonia + Pyruvicacid

H2S

Fe (Iron)

FeS (Ferrous Sulfide) Black

amino acid enzymes3
Amino Acid Enzymes

Lysine decarboxylase

Lysine Cadaverine + Carbon dioxide

lysine decarboxlyase broth
Lysine Decarboxlyase Broth
  • Lysine
  • Fermentable sugar
  • ph indicator
    • Basic = purple
    • Acid = Yellow
  • Durham Tube
  • Mineral Oil on top after inoculation
  • (maintain an Anaerobic environment)
amino acid enzymes4
Amino Acid Enzymes

Phenylalanine deaminase

Phenylalanine Phenylpyruvic acid + ammonia

slide12

Phenylalanine deaminase

Phenylalanine Phenylpyruvic acid + ammonia

FeCl2 (Ferric Chloride)

HCl

Green