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Deparaffinization with xylene and Hydration with ethanol - PowerPoint PPT Presentation


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Deparaffinization with xylene and Hydration with ethanol. Ag retrieval by heating in citrate buffer. Serum Blocking and A vidin /biotin Blocking. Primary Ab Incubation with anti- d esmin. Peroxidase Blocking with Hydrogen peroxide. Biotinylated Secondary Ab Incubation.

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Deparaffinization with xylene and hydration with ethanol

Deparaffinization with xylene and Hydration with ethanol

Ag retrieval by heating in citrate buffer

Serum Blocking and Avidin/biotin Blocking

Primary Ab Incubation with anti-desmin

Peroxidase Blocking with Hydrogen peroxide

BiotinylatedSecondary Ab Incubation

Detection with avidin -HRP

DAB Substrate Incubation

Counterstaining with Hematoxylin