Deparaffinization with xylene and Hydration with ethanol. Ag retrieval by heating in citrate buffer. Serum Blocking and A vidin /biotin Blocking. Primary Ab Incubation with anti- d esmin. Peroxidase Blocking with Hydrogen peroxide. Biotinylated Secondary Ab Incubation.
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Deparaffinization with xylene and Hydration with ethanol
Ag retrieval by heating in citrate buffer
Serum Blocking and Avidin/biotin Blocking
Primary Ab Incubation with anti-desmin
Peroxidase Blocking with Hydrogen peroxide
BiotinylatedSecondary Ab Incubation
Detection with avidin -HRP
DAB Substrate Incubation
Counterstaining with Hematoxylin