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Deparaffinization with xylene and Hydration with ethanol

Deparaffinization with xylene and Hydration with ethanol. Ag retrieval by heating in citrate buffer. Serum Blocking and A vidin /biotin Blocking. Primary Ab Incubation with anti- d esmin. Peroxidase Blocking with Hydrogen peroxide. Biotinylated Secondary Ab Incubation.

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Deparaffinization with xylene and Hydration with ethanol

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  1. Deparaffinization with xylene and Hydration with ethanol Ag retrieval by heating in citrate buffer Serum Blocking and Avidin/biotin Blocking Primary Ab Incubation with anti-desmin Peroxidase Blocking with Hydrogen peroxide BiotinylatedSecondary Ab Incubation Detection with avidin -HRP DAB Substrate Incubation Counterstaining with Hematoxylin

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