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TRACK NIGHT 2013

TRACK NIGHT 2013. Hosted by BMES UT Austin Student Chapter. Sign in at the front Please fill out a survey before you leave. TRACK NIGHT 2013. Hosted by BMES UT Austin Student Chapter. Sign in at the front Please fill out a survey before you leave. TRACK NIGHT 2013. Technical Area/Track 1.

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TRACK NIGHT 2013

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  1. TRACK NIGHT 2013 Hosted by BMES UT Austin Student Chapter • Sign in at the front • Please fill out a survey before you leave

  2. TRACK NIGHT 2013 Hosted by BMES UT Austin Student Chapter • Sign in at the front • Please fill out a survey before you leave

  3. TRACK NIGHT 2013 Technical Area/Track 1 Technical Area/Track 2 Technical Area/Track 3

  4. TRACK NIGHT 2013 Technical Area/Track 1 Technical Area/Track 2 Technical Area/Track 3

  5. Technical Area/Track 1 Biomedical Imaging and Instrumentation Focused on imaging, diagnostics, and therapy through biomedical instrumentation

  6. Track 1: Biomedical Imaging and Instrumentation Skills • Image and signal processing • Analog/digital network analysis • Software/hardware programming • Circuit Design • Data acquisition • Computational analysis of data in regard to living systems

  7. Track 1: Biomedical Imaging and Instrumentation E E 312 Software Design and Implementation I E E 438 Fundamentals of Electronic Circuits E E 445S Real-Time Digital Signal Processing Lab • Some Technical Electives • BME 357: Biomedical Imaging Modalities • EE 371R: Digital Image & Video Processing • BME 374K: Instrument Design • EE 445L: Embedded Systems Lab • EE 422C: Software Design & Implementation II • EE 347: Modern Optics • BME 347: Fundamentals of BME Optics + 4 hours of Engineering Electives

  8. Track 1: Biomedical Imaging and Instrumentation + 4 hours of Engineering Electives

  9. Track 1: Biomedical Imaging and Instrumentation Companies • National Instruments • Hospira • Flextronics • GE Healthcare • Medtronic • Biomet • Stryker • Arthrocare • Intuitive Surgical • Siemens Healthcare • Philips Healthcare • Zimmer • St. Jude Medical

  10. Track 1: Biomedical Imaging and Instrumentation Area Faculty • Andrew Dunn • StanislavEmelianov • Thomas Milner • H. Grady Rylander • Konstantin Sokolov • James Tunnell • Tim H.C. Yeh • XiaojingJohn Zhang

  11. Multi-Modal Spectroscopy for Early Cancer Detection • One project in our lab uses a combination of radiative and non-radiative techniques: • Multi-Modal Spectroscopy (MMS): Reflectance, Fluorescence, and Raman Spectroscopy • Multiple techniques at once provides complementary information • We aim to use an MMS approach for the early detection of skin cancer SpectroscopyPhysiology Raman Lipids Beta-carotene N/C ratio Light Scattering Microarchitecture Absorption Blood perfusion Hypoxia Water Fluorescence NADH, FAD Collagen Very good collection/excitation overlap

  12. Spectral Diagnosis System Spectrometer Optical switch BS light source Camera Optical fiber probe Xenon Lamp N2 Laser spectrograph Microcontroller Fiber-optic capture Spectrometer 1 mm 830nm laser Camera Source: BBC News, “Health contents” 2005 Rajaram Appl Opt 2011

  13. Probe Performance Probe Performance • Reflectance Optical Fibers D I1 S Io • Raman Tissue µs’ µa MM = malignant melanoma PL = pigmented lesion BCC = basal cell carcinoma SCC = squamous cell carcinoma AK = actinic keratosis AKSCC = AK + SCC N= normal

  14. Gold Nanoparticle-Mediated Imaging and Photothermal Therapy of Cancer Cells • Another project utilizes Gold Nanorods for Cancer Imaging and Therapy: • Image gold nanorods with two-photon microscopy utilizing a ultrafast fs pulsed laser to get high axial and lateral resolution • Photothermal therapy, taking advantage of gold nanorods passively and actively targeted to cancer cells/tumors and using the heat induced from gold nanorods when excited with laser at nanorod resonance frequency • Gold nanorod (GNR) properties: • Inert and biocompatible • Easily bioconjugable • Surface plasmon resonance: • Strong absorption and scattering cross-sections (imaging and therapy)

  15. Gold Nanoparticle-Mediated Imaging and Photothermal Therapy of Cancer Cells • Photothermal Therapy: • Irradiate with near-infrared laser • Induce heat • Measure cell damage Nanoparticles • Two-photon Imaging (Z-stacks): • PEG GNRs (red) are non-specifically bound to the membrane (green) of the cell • CTAB GNRs (red) are internalized within cells

  16. Gold Nanorod Uptake in Multicellular Tumor Spheroids 3D Cellular in vitro Models of Cancerous Tumors Optically Magnified Z-Stack 3D Reconstruction Green: Cell Membrane Stain Red: Gold Nanorods Blue: Nuclei

  17. Final thoughts on… Technical Area/Track 1 Biomedical Imaging and Instrumentation Classes, Opportunities, Post-Grad

  18. TRACK NIGHT 2013 Technical Area/Track 1 Technical Area/Track 2 Technical Area/Track 3

  19. Technical Area/Track 2 Cell and Biomolecular Engineering Integration of cell & molecular biology with engineering analysis; Address problems in molecular medicine

  20. Track 2: Cell and Biomolecular Engineering Skills • Tissue engineering • Materials science • Construction of nanoscale devices • Creation of bioactive materials • Bioengineering perspective on disease and immune response • Modeling biological systems

  21. Track 2: Cell and Biomolecular Engineering BME 352 Engineering Biomaterials BME 339 Biochemical Engineering BME 344 BME 354 BME 379 • Some Technical Electives • BME 342: Biomechanics of Human Movement • BME 344: Biomechanics • BME 354: Molecular Sensors & Nanodevices for BME Applications • BME 379: Tissue Engineering • Upper-Division Biology (Genetics) • Organic Chemistry II & Lab + 6 hours of Engineering Electives

  22. Track 2: Cell and Biomolecular Engineering + 6 hours of Engineering Electives

  23. Track 2: Cell and Biomolecular Engineering Companies • Genentech • Merck • Pfizer • Dow • Procter and Gamble • Boston Scientific • Osteomed • Biomet • Amgen • Life Technologies • GE • Terapio

  24. Track 2: Cell and Biomolecular Engineering Area Faculty • Aaron Baker • Amy Brock • George Georgiou • Jenny Jiang • Nicholas Peppas • Michael Sacks • Jeanne Stachowiak • Laura Suggs • Janet Zoldan

  25. Laboratory of Biomaterials, Drug Delivery and Bionanotechnology Nicholas A. Peppas

  26. ORAL PROTEIN DELIVERY

  27. Protein Therapeutics • Advantages • Complex, unique functions • Faster FDA approval • Over 130 approved protein therapeutics1 • In 2010, sales exceeded $93 billion2 • Disadvantages • High cost • Compound stability • Large molecular weight • Route of administration www.vivo.colostate.edu www.cs.stedwards.edu www.tarsatherapeutics.com 1 Leader, Benjamin et. al. Protein therapeutics: A summary and pharmacological evaluation. Nat Rev. Drug Disc. 7 (2008) 21-39. 2 Maheshwari, Shushmul. Global protein therapeutics market: Beefing up towards futuristic growth.

  28. Oral Delivery of Therapeutic Proteins • Benefits • Ease of administration • Increased patient compliance • Lower cost • Challenges • Maintaining stability and activity in the stomach • Narrow absorption window in the small intestine • Transport across endothelium to the bloodstream

  29. pH-Responsive Systems Complexed; small mesh sizepH ~2 x Decomplexed; increased mesh size pH ~5-7 x

  30. Promoting Particle Interaction with the Environment Tether-containing polymer/ drug complexes for mucoadhesion, which have an increased residence time Tether-containing polymer/ drug complexes for mucoadhesion, which have an increased residence time Tether-containing polymer/ drug complexes for mucoadhesion, which have an increased residence time Caco-2 cells with tight junction stained Caco-2 cells with tight junction stained Caco-2 cells with tight junction stained Caco-2 cells as a gastrointestinal model Caco-2 cells as a gastrointestinal model Caco-2 cells as a gastrointestinal model

  31. Oral Chemotherapeutic Delivery

  32. C=O ( CH2 - CH2 – O ) . . . Overcoming the challenges of oral chemotherapeutic delivery Sequential Interpenetrating Polymer Network Grafted, Copolymer Network Grafted PEG Chains Hydrophobic Components Graft Hydrophilic Network Hydrophobic Nanoparticles embedded in Hydrophilic Network 275 nm hydrophobic nanoparticles OH

  33. ORAL siRNA DELIVERY

  34. A combined approach to oral siRNAdelivery Polycationic polymers mediate endosome disruption through the proton sponge mechanism. Following endosomal escape, entrapped biomolecules can be released into the cytosol. Fluorescently-labeled nanogels after cellular uptake PLGA micro-particles Fluorescently-labeled nanogels after cellular uptake

  35. MOLECULAR RECOGNITION

  36. Functional Monomers Crosslinker Template Molecule Extraction and drying Components Complexation Solvent Initiator Recognitive Cavity Polymerization Molecularly Imprinted Polymer (MIP) Methodology • Used for diagnostic platforms

  37. Bionanotechnology

  38. Hydrogels and Heat • Gold-polymer nanoparticle for triggered and targeted drug delivery • Upon laser irradiation, a thermo-sensitive polymer collapses to release therapeutics. • Real time imaging via ultrasound/photoacoustic methods. Temperature sensitive nano-composites with Fe3O4 and gold NPs shown schematically, with electron microscopy, and thermal-IR imaging.

  39. Final thoughts on… Technical Area/Track 2 Cell and Biomolecular Engineering Classes, Opportunities, Post-Grad

  40. TRACK NIGHT 2013 Technical Area/Track 1 Technical Area/Track 2 Technical Area/Track 3

  41. Technical Area/Track 3 Computational Biomedical Engineering Use of computational algorithms to address problems in healthcare and biomedical research

  42. Track 3: Computational Biomedical Engineering Skills • Design medical decision aids • Dynamic analysis of biomechanics • Thermodynamic modeling of bio-molecular reactions • Image processing and interpretation • Computational genomics

  43. Track 3: Computational Biomedical Engineering E E 360C Algorithms E E 312 Software Design and Implementation I M 325K Discrete Mathematics E E 422C Software Design and Implementation II • Some Technical Electives • BME 341: Engineering Tools for Computational Genomics Lab • BME 342: Biomechanics of Human Movement • BME 344: Biomechanics • BME 345: Graphics and Visualization • BME 346: Computational BiomolecularEngineering • M 340L: Matrices and Matrix Calculations + 5 hours of Engineering Electives

  44. Track 3: Computational Biomedical Engineering + 5 hours of Engineering Electives

  45. Track 3: Computational Biomedical Engineering Companies • Ortho-Kinematics • Luminex • AssureRX • Diagnostic Hybrids • Proscan Imaging • Novartis • IBM • Pfizer

  46. Track 3: Computational Biomedical Engineering Area Faculty • Kenneth Diller • Mia Markey • PengyuRen • Michael Sacks

  47. Track 3: Computational Biomedical Engineering To develop a model of the mitral valve that will optimize the restoration of homeostatic normal tissue stress 3D Reconstruction of MV based on Micro-CT images1 • Mitral valve (MV) repair: annuloplasty rings • Recurrence of mitral regurgitation after repair due to excessive tissue stress • Multiscale model: valve geometry, tissue stress, MV interstitial cells 1. Lee, Chung-Hao et al.2013

  48. Track 3: Computational Biomedical Engineering Generation of 3D Finite Element Model for MV & Estimated Leaflet Thickness based on Micro-CT Images1 1. Lee, Chung-Hao et al.2013

  49. Track 3: Computational Biomedical Engineering To create a 3D rendition of the MVIC microenvironment using electron microscopy techniques • TEM or SEM • 3D reconstruction software: • Turboreg, Stagreg • Reconstruct • Blender • Amira • Heymann: 2D & 3D imaging of yeast cells Heymann et al. 2006

  50. Thoughts on… Technical Area/Track 3 Computational Biomedical Engineering Classes, Opportunities, Post-Grad

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