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Expression and Regulation of AIP in Mouse and Human T Cells

This study investigates the expression of AIP in primary mouse and human T cells through western blotting and RNA analysis in response to CD3/CD28 stimulation.

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Expression and Regulation of AIP in Mouse and Human T Cells

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  1. B C A Mouse 2 Mouse 3 Mouse1 Donor 2 Donor 1 Donor 3 AIP 35- 35- p65 65- AIP 65- p65 Additional file 2AIP is expressed in primary mouse and human T cells. (A) AIP is expressed in primary human T cells. T cells of three human donors were isolated and cell lysates analyzed by western blotting. (B) AIP is expressed in primary mouse T cells. T cells of three mice were isolated and cell lysates analyzed by western blotting. (C) AIP mRNA levels do not significantly change in response to CD3/CD28 stimulation. T cells of three mice were isolated and CD3/CD28 stimulated for the indicated time points. RNA was isolated and AIP transcript levels analyzed by quantitative RT-PCR. Bars show average and standard deviation of three analyzed mice. Additional file2

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