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Analysis of CRTC2 phosphorylation via immunofluorescence in WT and mutant cell lines

This figure shows CRTC2 localization and phosphorylation status in different cell lines through immunofluorescence staining. Wild-type cells as well as cells with S171A and S275A mutations were analyzed. The merge images demonstrate the impact of forskolin treatment on CRTC2 localization.

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Analysis of CRTC2 phosphorylation via immunofluorescence in WT and mutant cell lines

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  1. Additional file 2: Figure S2 A CRTC2 FSK CRTC2/FLAG DAPI Merge – WT + WT S171A S275A – B pCRTC2-S171 (80 kDa) CRTC2 (80 kDa) β-actin (42 kDa) – + – + – + FSK Vector CRTC2 CRTC2 (S171A)

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