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LECTURE PROPER. VECTOR. An arthropod (insects, mites, ticks) that are capable of: acquiring, supporting the development and transmitting a pathogenic agent (bacteria, viruses, parasites) from one host to another host. Ex: mosquito Aedes aegypti transmitting dengue virus. VECTOR CONTROL.
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VECTOR • An arthropod (insects, mites, ticks) that are capable of: acquiring, supporting the development and transmitting a pathogenic agent (bacteria, viruses, parasites) from one host to another host. Ex: mosquito Aedes aegypti transmitting dengue virus
VECTOR CONTROL • suppression of a target population by using measures to alter the vector reproductive capacity or potential (Vector Genetics Course, 2010, India). NET REPRODUCTIVE RATE (NRR) and GROSS REPRODUCTIVE RATE (GRR)
NET REPRODUCTIVE RATE (NRR) • refers to the survival of eggs of mosquitoes to adults (number of eggs surviving to become adults) • dependent on the following: 1. number of containers available for Aedes vectors to lay their eggs
NET REPRODUCTIVE RATE (NRR) • dependent on the following: 2. condition of the containers; can hold water for at least seven (7) days; covered; uncovered 3. interspecies competition Ae. aegypti vs Ae. Albopictus
NET REPRODUCTIVE RATE (NRR) • dependent on the following: 4. Predators 5. Larvicides 6. Larval food; temperature for pupal development
NET REPRODUCTIVE RATE (NRR) EX: 100 eggs laid on the trap/any container How many will emerge as adults? 0; 25; 50; 100?
GROSS REPRODUCTIVE RATE (GRR) • refers to the number of adult females surviving through one day to lay their eggs • influenced by: 1. female adult completion of gonotrophic cycle 2 to 3 days (> 250C); obtaining their blood meal and laying their first batch of eggs
GROSS REPRODUCTIVE RATE (GRR) • influenced by: (early death within a day; prevents an adult female to mate with adult males) • no mating; no blood meal; no laying of eggs • this is where adulticides are useful
GROSS REPRODUCTIVE RATE (GRR) EX: 100 eggs laid on the trap/any container 50 emerged as adults 25 died on day 1 25 survived and successfully mated 20 died on day 2 only 5 survived on day 3 and fed on humans
DENGUE VECTOR CONTROL Application of various anti-DEN mosquito measures to alter the NRR and GRR
DENGUE VECTOR CONTROL • Specific targets: • Prevent DEN transmission and cut DEN transmission through: 1. lowering the NRR by - trapping(ovitrapping/ OLtrapping ) - larviciding - container management - environmental sanitation
DENGUE VECTOR CONTROL • Specific targets: through: 2. lowering the GRR by: - adulticiding (space-spraying; aerosol treatment) - genetic manipulation of the reproductive capacity of the mosquito
DENGUE VECTOR CONTROL • Specific targets: through: 3. prevention and man-mosquito contact - curtains - long trousers - repellents
INTEGRATED (DEN) VECTOR CONTROL integration/combination of measures to reduce the NRR and GRR
INTEGRATED (DEN) VECTOR MANAGEMENT • utilization of technical and managerial measures to bring about an effective degree of vector suppression to prevent and control DEN transmission Key words: effective degree of vector suppression
COMPONENTS OF IVC • Vector biology/behavior – Aedes DEN vectors • Surveillance tool; ovitrap/oltrap Surveillance index 3. Critical Threshold Density Index(ces) or effective degree of vector suppression (OL Index) 4. Control measures 5. Monitoring and Evaluation
WHAT : (Mosquito and Dengue) Biology 1.1 Aedes mosquito vectors a. Aedes aegypti b. Aedes albopictus
Vector Characteristics • Biting Habits • “daybiters” with two peaks of biting time: • 1 to 2 hours after sunrise • 1 to 2 hours before sunset • only female mosquitoes bite because of the requirement of blood for oviposition • males do not bite but feed on plant juices and nectar of flowers
Vector Characteristics • Flying Habit • do not fly great distances • farthest flight distance is within 200 to 400 meters radius from their breeding places • Adults found around 50 meters from the breeding sources
Bionomics • at temperatures <10oC and >40oC, no developmental process takes place • female mates with male only once • oviposition takes place about 2 to 5 days from blood engorgement • prefers dark-colored background for oviposition • lays egg 3 to 4 times in its lifetime • 60 to 100 eggs per batch
Life Cycle (10-12 days) Adult -Wings uniformly grayish black -Body and legs are black with distinctive white patches throughout -Thorax has markings Eggs: - Black and oval in shape - Laid singly above water surfaces of containers 2 - 3 days 1 - 2 days Larva: - Feeding stage - Breeds in clean and non-polluted water - Rests at an angle to the water surface Pupa -Non-feeding stage -Breeding trumpet is long, slender with narrow opening 6 - 8 days