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Understanding DNA Replication Mechanism and Repair Processes

Explore the intricate process of DNA replication, from nucleoside polymerization to repair mechanisms like mismatch repair and recombination. Learn about the enzymes involved, such as DNA polymerase and DNA ligase, and how base flipping influences genetic recombination and repair. Discover the initiation of replication and the role of accessory proteins in ensuring accurate DNA synthesis.

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Understanding DNA Replication Mechanism and Repair Processes

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  1. DNA REPLICATION

  2. Nucleosid Nucleotid & Nucleosid

  3. Figure 07.08

  4. Figure 07.09

  5. Figure 07.18

  6. Polymerization of the deoxynucleotides during DNA synthesis. The β and γ phosphates of each deoxynuleosid triphosphate are cleaved off to give energy for the polymerization reaction

  7. A single base can be flipped out from the double helix,which could be important in recombination and repair

  8. Figure 01.11

  9. Figure 09.23

  10. DNA polymerase { ΙΙΙ , Ι } • Nuclease { endonuclease , exonuclease [ 5´,3´ exonuclease]} • DNA ligase • Primase • Accessory proteins : 10 different protein: β clamp → dnaN gene , clamp loader → help the β clamp • Helicases and helix- desabilizing proteins or single strand DNA binding proteins • Topoisomerases

  11. Figure 09.18

  12. ori C

  13. Initiation of replication at E.coli oriC

  14. DNA Repair • Mismatch repair • Excision repair • Recombination repair • SOS repair

  15. Mismatch repair(methyl-directed mismatch repair)

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