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Cryopreservation of Giraffe Epididymal Sperm Cells: Evaluating Recovery and Viability

This study aims to assess the sensitivity of giraffe (Giraffa camelopardalis) epididymal sperm cells to cryopreservation techniques. Sperm samples were collected post-mortem and prepared for cryopreservation using two different extenders, Gent B and Gent A mixed with varying DMSO concentrations. The evaluation involved analyzing total and progressive motility, sperm viability, morphological abnormalities, and acrosome integrity through advanced methods. Results indicate successful post-mortem recovery, with optimal cryopreservation observed using the GA + 5% DMSO extender. This advances our understanding of reproductive biotechnologies in wildlife conservation.

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Cryopreservation of Giraffe Epididymal Sperm Cells: Evaluating Recovery and Viability

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  1. RESULTS MATERIAL & METHODS CRYOPRESERVATION OF GIRAFFE (Giraffacamelopardalis) EPIDIDYMAL SPERM CELLS Maya-Soriano MJ1, Fernández-Bellón H2,Taberner E1, Mayor P1, AlmagroV2, Enseñat C2, López-Béjar M1 1Department of Animal Health and Anatomy, Faculty of VeterinaryScience, UniversitatAutònoma de Barcelona, 08193 Bellaterra, Barcelona, SPAIN; mariajose.maya@uab.cat 2ParcZoològic de Barcelona, Parc de la Ciutadella s/n, 08003 Barcelona, SPAIN. The aim of this study was to evaluate the sensitivity of giraffe (Giraffacamelopardalis) epididymal sperm cells to cryopreservation. Sperm samples were obtained within 24h post-mortem after flushing caudaeepididymaand deferent ducts with Kenney’s medium. Sperm sample recovery (10 ml, 500 x 106 spz/ml) Samples were evaluated at recovery and prepared for conventional cryopreservation by using two different extenders: commercial Gent B (Minitüb) or commercial Gent A (Minitüb) mixed with DMSO at different concentrations (1, 5 and 10%). Evaluation of spermparameters Total and progressivespermmotilities (CASA system) Sperm viability and total morphological abnormalities (eosin-nigrosin staining) Acrosome integrity (FITC-PSA lectin staining) 2cm Table 1. Parameters of epididymal sperm cells at recovery (0h) and after cryopreservation. Percentages based on analysis of at least 200 sperm cells per sample.A high percentage of sperm cells showed cytoplasmaticdrops. Figure 1. Sperm cells with altered (A) and intact acrosome (B) membrane after FITC-PSA lectin staining (green fluorescence) and DAPI nuclear staining (blue fluorescence). CONCLUSION Post-mortem sperm cell recovery and cryopreservation is possible in the giraffe and it can be suggested that an appropriate extender in this study was “GA + 5% DMSO”.

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