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Biochemistry 482 Amino Acid metabolism. Biochemistry 482 Amino Acid metabolism. Biochemistry 482 Amino Acid metabolism. Biochemistry 482 Amino Acid metabolism. Biochemistry 482 Amino Acid metabolism. Biochemistry 482 Amino Acid metabolism. Biochemistry 482 Amino Acid metabolism.

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biochemistry 482 amino acid metabolism9
Biochemistry 482 Amino Acid metabolism

From GluN

From OAA & GluN

From GlnNN

biochemistry 482 amino acid metabolism11
Biochemistry 482 Amino Acid metabolism

This is an allosteric

Activator of

CPS-1

biochemistry 482 amino acid metabolism12
Biochemistry 482 Amino Acid metabolism

Supplies NH3

Activation to give good LG

biochemistry 482 amino acid metabolism13
Biochemistry 482 Amino Acid metabolism

Activation Nu Subst @ >C=N+

Arg is a good leaving group

and mechanism

is via E1CB to give fumarate

and Arg

tetrahydrofolates thfs
Tetrahydrofolates THFsBiochemistry 482 Amino Acid metabolism

One carbon transfer via THFs

From dietary ‘folate’ and 2NADPHs

nitrogen fixation
Nitrogen fixationBiochemistry 482 Amino Acid metabolism

N2 + 8H+ + 8e- + 16ATP + 16H2O --->2NH3 + H2 + 6ADP + 16 Pi

biochemistry 482 amino acid metabolism20
Biochemistry 482 Amino Acid metabolism

ATP drives endothermic electron transfer to eventually N2