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The aim of our work was to analyze subtype specific immune responses

Characterization of vaccine-vectors expressing Nef of the BF recombinant HIV-1 circulating form and evaluation of the immune response induced in mice.

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The aim of our work was to analyze subtype specific immune responses

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  1. Characterization of vaccine-vectors expressing Nef of the BF recombinant HIV-1 circulating form and evaluation of the immune response induced in mice AM.Rodríguez1, G.Schulman1, MF.Pascutti1, F.Ferrer2, G.Turk1, JL. Najera3, D.Mónaco1, G.Calamante2, M.Esteban3, H.Salomón1 and MM.Gherardi1 1Centro Nacional de Referencia para el SIDA, Universidad de Buenos Aires, Buenos Aires, Argentina 2Instituto de Biotecnología,CICVyA-INTA Castelar, Buenos Aires, Argentina 3Departamento de Biologia molecular y Celular, Centro Nacional de Biotecnologia, CSIC, Madrid, España INTRODUCTION In Argentina, epidemiological studies revealed that early predominance of B subtype has diminished due to the emergence of BF recombinants The aim of our work was to analyze subtype specific immune responses For this we developed vectors expressing Nef from CRF12_BF MVA Vector Characterization DNA and MVA VectorsConstruction DNA vector MVA vector DNA Vector Characterization

  2. Cellular immune responses ELISPOT ELISA IFN-g (pg/ml) IFN-g SFC/106 Cross-reactivity against NefB ELISA IFN-g (pg/ml) Conclusion • We developed and characterized DNA and MVA vectors expressing NefBF. • We demonstrated the ability of these vectors to express Nef from murine and human cells. • Both vectors were immunogenic in mice, with low cross-reactivity against B subtype. This work is relevant for the future design of HIV vaccines in our country where there is a high prevalence of BF recombinant form.

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