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Real-Time PCR Analysis of HLA-A, HLA-B mRNA Copy Number: Control and IFN-g Induction

This study examines mRNA copy numbers of HLA-A and HLA-B genes under control and IFN-g induced conditions using real-time PCR. The findings shed light on constitutive and inducible gene expression patterns.

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Real-Time PCR Analysis of HLA-A, HLA-B mRNA Copy Number: Control and IFN-g Induction

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  1. 25 HLA-A/GUSB 20 15 10 5 0 E-004 E-159 Control positive + IFN-g E-159 + IFN-g E-004 + IFN- g A Real-time PCR 40 HLA-B/GUSB 35 30 25 HLA-A mRNA copy number/ GUSB mRNA copy number HLA-B mRNA copy number/ GUSB mRNA copy number 20 15 10 5 0 E-004 E-159 E-159 + IFN-g Control Positive Positive Control + IFN-g E-004 + IFN- g Positive Control B IFN-g Flow Cytometry IFN-a HLA-ABC Negative control HLA-b2m HLA-ABC HLA-b2m ESTDAB-004 counts counts counts Constitutive expression HLA-ABC HLA-b2m HLA-b2m HLA-ABC counts counts counts Inducible expression ESTDAB-159 FL1-Height FL1-Height FL1-Height FL1-Height

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