'Pcr reaction' presentation slideshows

Real-Time PCR

1.71k views • 82 slides

Real-Time PCR

David A. Palmer, Ph.D. Technical Support, Bio-Rad Laboratories Adjunct Professor, Contra Costa College. Real-Time PCR. Objectives. This presentation will cover the following topics: What is real-time PCR used for? How does real-time PCR work? What instruments are used?

1.08k views • 79 slides

Real-Time PCR

David A. Palmer, Ph.D. Technical Support, Bio-Rad Laboratories Adjunct Professor, Contra Costa College. Real-Time PCR. Objectives. This presentation will cover the following topics: What is real-time PCR used for? How does real-time PCR work? What instruments are used?

1.32k views • 74 slides

An Overview of the PCR

788 views • 62 slides

HIV Drug Resistance Training

HIV Drug Resistance Training. Module 3: Principles of PCR and HIVDR Sequencing. Topics. Isolation and Amplification of DNA Sequencing Identifying Mixtures Lab Procedures. Objectives. Describe the PCR process.

780 views • 49 slides

Lecture 28

Lecture 28. ?? D ???? D.1 DNA ???? D.2 ????? D.3 ???? ??????. Appendix D Experimental Techniques D.1 DNA manipulation techniques D.2 Manipulating Proteins D.3 The Function of Genes and their Proteins. Appendix D Experimental Techniques / ????.

682 views • 46 slides

Molecular SEQUENCE DATA Generation - From template DNA to Sequence Alignment or other stuff

Molecular SEQUENCE DATA Generation - From template DNA to Sequence Alignment or other stuff. Protocol. Collect tissue samples from whatever and extract tDNA Amplify specific gene using PCR (Polymerase Chain Reaction) Sequence PCR products Align our sequence with published sequences

632 views • 42 slides

mRNA Quantification

mRNA Quantification. Real-time PCR. To accurately quantify starting amounts of nucleic acid during the PCR reaction without the need for post-PCR analyses. A fluorescent reporter is used to monitor the PCR reaction as it occurs; the reporter can be of a nonspecific or specific nature.

482 views • 34 slides

tools for Molecular Biology Amplification

tools for Molecular Biology Amplification. The PCR Reaction. The PCR reaction is a way to quickly drive the exponential amplification of a small piece of DNA. PCR is a 3 step process Denaturation of the target DNA Annealing of your gene specific primers

506 views • 32 slides

Cloning and Sequencing Explorer Series

Cloning and Sequencing Explorer Series. ??? ? ? ? ?. Related knowledge. Molecular cloning Plasmid/vector DNA sequencing technology DNA sequence analysis.

645 views • 32 slides

Diagnosing Niemann Pick disease, Type C

Diagnosing Niemann Pick disease, Type C. MODIFIED FROM A SLIDE SHOW Developed by the Sanford PROMISE. The Sanford PROMISE Program for the Midwest Initiative in Science Exploration. The Case.

783 views • 31 slides

Peter F. Straub, Professor of Biology, DSO Tara Harmer, Assistant Professor of Biology

Distribution of the Northern Star Coral ( Astrangia poculata ) and its Symbiotic Zooxanthellae on New Jersey Artificial Reefs. Peter F. Straub, Professor of Biology, DSO Tara Harmer, Assistant Professor of Biology School of Natural Sciences & Math Richard Stockton College of NJ.

415 views • 25 slides

Listeria monocytogenes

Listeria monocytogenes. Listeria monocytogenes. Invasive pathogen WEAR GLOVES!!! Listeriosis YOPI Miscarriage, stillbirth, premature birth Bacteremia Meningitis Meningoencephalitis. Location of L. monocytogenes. Ubiquitous Raw foods Processed RTE foods Food processing environment.

2.47k views • 18 slides

PCR AND PRIMER DESIGN

405 views • 16 slides

Gel Interpretation & DNA Clean-up

Gel Interpretation & DNA Clean-up. Tucson High School Biotechnology Course Spring 2010. What have we done so far?. negative control. psbA. virus DNA. positive control. negative control. DNA pol. virus DNA. positive control. negative control. g23. virus DNA. positive control.

190 views • 9 slides

PROBLEM:

PROBLEM: At the time there was never enough DNA in any experiment, i.e., it was always limiting, and whatever human DNA there was in an experiment was hopelessly contaminated with itself.

159 views • 7 slides

Diagnosis with PCR

Diagnosis with PCR. This is a preparation of DNA . We zoomed in a portion of a gene. We know that two primers, Forward and Reverse , will hybridize at specific places in this exon. During a PCR reaction , a fragment of definite size will be amplified. Diagnosis with PCR.

226 views • 5 slides

201 views • 1 slides

ICAM-1

(B). (A). 10 -14. 10 -13. 10 -11. 10 -12. 10 -10. 1x. 0.1x. 0.01x. 0.001x. IS conc. (M). ICAM-1 conc. (fold). ICAM-1. ICAM-1. IS (10 -14 M). IS. -10. 0.0. -11. -0.5. -1.0. -12. Log 10 (IS conc.). Log 10 (ICAM-1 conc.). r 2 =0.9861. -1.5. r 2 =0.9913. -13. -2.0. -14.

132 views • 1 slides

Introduction

259 views • 1 slides