Sensitization and Agglutination. Antigen-Antibody reaction. Red cell Ag-Ab reaction can be detected by a number of techniques Most frequently used Hemolysis Occurs if the entire complement sequence is activated following Ag-Ab interaction Frequently stops at C3 – no lysis
adacent red cells forming clumps.
This represents what occurs during stage one of agglutination.
This represents what occurs during stage 2 of agglutination:
Antibody molecules crosslink RBCs forming a lattice that results in visible clumping or agglutination.
1- Ratio of Ab to Ag
4- Ionic strength of the medium
1- Ionic strength of Medium
2-Presence of Albumin in medium
3- Enzyme treatment of red cells
4- Temperature (previously discussed)
6- Ag Clustering and Mobility
Clustering of Ags