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Electrophoresis and Electrolysis in Chemistry

Explore the principles of electrophoresis and electrolysis, the role of electric fields and currents, and the importance of gel structures. Learn about DNA electrophoresis and SDS-PAGE techniques in this comprehensive guide.

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Electrophoresis and Electrolysis in Chemistry

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  1. Chemistry in action….Or….Ask your friendly chemist…

  2. Chemistry of electrophoresis and electrolysisElectric fieldsand electric currents

  3. SDS-PAGE, DNA electrophoresis and the need for gel (sieving medium)Without a viscous medium, all move at ~the same rate in electric fieldGel structure serves to retard larger solute particlesDNA molecules, SDS proteins have ~equal charge/massElectrophoresis occurs between the electrodes (field-driven).

  4. Electrolysis always occurs during electro-phoresis.Cathode produces H2 at twice the rate that anode produces O2Current is carried by solute ions. Electrons aren’t soluble in H2O.Example: TAE buffer; tris suppliescations(+),acetatesupplies anions(-).Electrolysis occursat the electrodes.

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