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Protists I

Protists I. Lab 3 BIOL 171. Remember!: Classification System. We’ll be looking at all of these! P rotists are everywhere in Eukarya ! “the junk drawer of the eukaryotes”. Ancestral Eukaryote. We’ll be looking at all of these! P rotists are everywhere in Eukarya !

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Protists I

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  1. Protists I Lab 3 BIOL 171

  2. Remember!: Classification System

  3. We’ll be looking at all of these! Protists are everywhere in Eukarya! “the junk drawer of the eukaryotes” Ancestral Eukaryote

  4. We’ll be looking at all of these! Protists are everywhere in Eukarya! “the junk drawer of the eukaryotes” Ancestral Eukaryote

  5. 6 Kingdoms • Plants (Plantae) • Animals (Animalia) • Fungi (Fungi) • Eubacteria • Archaeabacteria • Protista

  6. A constantly changing system…

  7. Lab Study A: Euglenozoans • Trypanosomalevisi(prepared slide) • Euglena (make wet mount) – not in manual (use depression slide) • Termites (Trichonympha) - procedure not in manual

  8. Trypanosoma and red blood cells

  9. Euglena

  10. Trichonympha • Lives in the intestine of the termite • Bacterial endosymbionts inside Trichonympha digest cellulose - Termite > Trichonympha > Spirochetes Procedure • Place a couple of drops of Ringer’s solution on a clean slide. • Transfer a termite into the drop of solution. • Place slide under a dissecting microscope. • Place the tips of dissecting needles at either end of the termite and pull in opposite directions. • Locate the long tube that is the termite’s intestine. • Place a cover slip over the specimen and lightly press down on coverslip to release the Trichonympha from the intestines. Observe with a compound microscope.

  11. Lab Study B: Alveolates Ciliate: Paramecium caudatum – (wet mount) in manual Dinoflagellates: mixed dinoflagellates (live & wet mount), and Peridinium (wet mount) not in manual

  12. Paramecium structures

  13. Dinoflaggelates

  14. Lab Study C: Stramenopiles Diatoms (Bacillariophyta) – make wet mount Also observe diatomaceous earth (the cell wall deposits from diatoms) – make wet mount and look at prepared slides

  15. Diatom diversity

  16. Diatom cell wall made of silica

  17. Stramenopile flagella

  18. Brown Algae (Phaeophyta) Living: Ectocarpus and Sphacelaria Preserved: Fucus and Laminaria

  19. Lab Study D: Rhizaria(different title from manual) • Foraminiferans - prepared slides

  20. Radiolarians - prepared slides

  21. Think about… • Morphological characteristics • Ecology of the organism • How does the organism get around? • What role do they play in the ecosystem? • Do they have any economic value? • Where do they live? • Don’t know the answer?? It’s probably a great research question! Ask me about it.

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