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This study investigates the dynamic interplay between HSF1 and p63 in gene expression control. Experimental data on HSF1 and p63 interactions through pull-down assays and ChIP analysis are presented, shedding light on their collaborative roles in transcriptional regulation.
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IP Ab HSF1 we Irr p63 HSF1 Int. I II III IV V F/l Pro 1-292 248-393 1-42 TAD DBD Oligo + + 248-393 1-42 1-292 Pro GST Inputs F/l + - - Pull down Supplementary Figure 1 Logan et al Supplementary Figure 2 Logan et al
p21 WAF1 10 9 8 7 Relative quantity -p53 6 + p53 5 4 3 2 1 0 PG13 MG15 p21-luc Relative change unt etop actD dox 12 10 8 6 4 2 0 Supplementary Figure 3 Logan et al Supplementary Figure 4 Logan et al
Supplementary Figure 5 Logan et al gadd45 HSF1 55 p53 45 irr 35 25 Relative recruitment 5 4 3 2 1 0 unt dox etop actD gadd45 100 HSF1 ChIP 80 p53 ChIP 60 Relative recruitment 40 20 0 siRNA p53 HSF1 ns Supplementary Figure 6 Logan et al
Unt HSF1 +/+ dox Unt HSF1 -/- dox p53 ser15 DAPI Supplementary Figure 7 Logan et al