Cellular Changes in E-Cadherin and Transferrin Fusion at Elevated Temperature

Mar 02, 2025

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This study examines the effects of merging E-cadherin and transferrin at 35°C for 4 hours. Supplementary Figure 1 from Palacios et al. shows the progression at 0, 4, 8, and 12 hours after v-Src activation. The extracellular domain epitope and cytoplasmic domain epitope are analyzed. The anti-E-cadherin response is monitored, as shown in Supplementary Figure 2 of Palacios et al.

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Cellular Changes in E-Cadherin and Transferrin Fusion at Elevated Temperature

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E-cadherin Transferrin Merge 35°C/ 4hrs Supplementary figure 1 Palacios et. al
0 4 8 12 0 4 8 12 Hours of v-Src activation Extracellular domain epitope Cytoplasmic domain epitope { Anti-E-cadherin Supplementary figure 2 Palacios et. al

Supplementary Figure 1 Abu Jawdeh et al.

Supplementary Figure 1 Abu Jawdeh et al. Quantitation of cNHE1 binding by densitometry, as determined by anti-6xHis antibody detection. Data are normalized to PI(4,5)P2 (defined as 1.0 in each experiment), and expressed as mean ± SEM. . Supplementary Figure 2 Abu Jawdeh et al.

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Rafehi et. al., Supplementary Figure 1

B. Rafehi et. al., Supplementary Figure 1. A. C. Rafehi et. al., Supplementary Figure 2. Rafehi et. al., Supplementary Figure 3. Keratinocytes (20 µM). K562 (100 µM).

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Mertens et al., Supplementary Figure

Mertens et al., Supplementary Figure.

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Supplementary Figure 1 Hori et al.

Preparation of LB-TT. Add 4 g CHAPS in a 300 (or 500) mL very clean/sterile Glass Beaker (calibrated if possible) . D issolve in 50 mL MQ water by slowly shaking using wrist movement; or better use a stirrer to mix, not to strongly…

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Supplementary Figure 1 Lewis, et al.

Cell Death (Ramos). Untreated. Proliferation (Ramos). hIgG (crosslinked). Dacetuzumab (soluble). Dacetuzumab (crosslinked). Percent Cell Death (apoptotic + necrotic). Crosslinking Ab. Dacetuzumab (soluble). hIgG (crosslinked). Dacetuzumab (crosslinked). CD40 Ligand.

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Nakayama et al. , Supplementary Figure 3

Nakayama et al. , Supplementary Figure 3. HNF4G/ACTB (microarray). HNF4G/ACTB (qRT-PCR). 0.035. 2.0. 0.030. 1.5. 0.025. 0.020. 1.0. 0.015. 0.010. 0.5. 0.005. 0.000. 0.0. SS043. SS043. SS043. SS043. SS043. SS043. SS046. SS046. SS046. SS046. SS046. SS046. SS049. SS049.

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Andrade et al. - Supplementary Figure 1

Andrade et al. - Supplementary Figure 1. A. 10 20 30 40 50 60 70 80 90 100 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

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Perugini et al Supplementary Figure 1

Accession. Gene Symbol. Gene Name. Direction of. Number. Regulation. Commonly Regulated. by FLT3. -. -. -. ITD (. Mizuki. , Tam) and GMR. -. -. -. V449E. (Brown). NM_009895. Cish. Cytokine inducible SH2-containing protein. . . . . . . NM_009982. Ctsc.

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Farmer et al Supplementary Figure 1

Farmer et al Supplementary Figure 1. [ KU0058684] (  M). 1. 2. 0. 1. 0. 0. IC 50 = 1nM. 0.00001. 0.0001. KU0051529 PARP-1 IC 50 = 730nM. 0.001. 0.01. 8. 0. 0.1. 100. 10. 0. 1. % activity. 6. 0. 4. 0. 2. 0. 0. 10 -6. 10 -5. 10 -4. 10 -3. 10 -2. 10 -1. 10 0.

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Supplementary Figure 1. Suzuki et al.

BrdU. DAPI/BrdU. 24 hrs. 24 hrs. 48 hrs. 48 hrs. 72 hrs. 72 hrs. Supplementary Figure 1. Suzuki et al. A. B. Supplementary Figure 2. Suzuki et al. p. CPD. H2AX. Merge. p. (6-4) photo. H2AX. Merge. p. XRCC1. H2AX. Merge. Supplementary Figure 3. Suzuki et al. No UV. 3 min.

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Oka et al. supplementary Figure 1 Top

Oka et al. supplementary Figure 1 Top. DAPI. Gr-1. MERGE. Supplementary Fig. 1. Identification of neutrophils by immunostaining with anti-Gr-1 antibody. Representative specimens of the UVB-irradiated skin of PLC  +/+ mice at 24 h

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Supplementary Figure 1 Logan et al

IP Ab. HSF1. we. Irr. p63. HSF1 Int. I. II. III. IV. V. F/l.  Pro. 1-292. 248-393. 1-42. TAD DBD Oligo. +. +. 248-393. 1-42. 1-292.  Pro. GST. Inputs. F/l. +. -. -. Pull down. Supplementary Figure 1 Logan et al.

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Supplementary Figure 1 – Toh et. al.

12 hr. 24 hr. Taxol:. 0 hr. 24 hr + PurvA. Time (hrs): -24 0 12-15 24-27. Cell Cycle Phase: Asyn. G1/S Mitosis. Thymidine block. Thymidine Wash-out; +/-Taxol;

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Oiva et al., Supplementary Figure.

Oiva et al., Supplementary Figure. P < 0.0001. 1000. P < 0.0001. 100. Trypsinogen-3 (  g/L). 10. 1. 0.1. Mild AP. Severe AP. Controls.

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Supplementary Figure 5. Nikolova et al

Supplementary Figure 5. Nikolova et al.

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Kim et al., Supplementary Figure 1.

Fig. S1. Effect of salt stress on OsCYP20-2 ectopic expression transgenic plants. a. *. *. *. *. V1 V2 T2 T3 T5. b. 0 50 100 150. V1 V2 T2 T3 T5.

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Leimgruber et al. Supplementary Figure 1

A. TSS. S’-Y’. S-Y. B. - IFN g. 1. Resistance to MNAse. 0.1. 0.01. units:. 0. 1. 2. 4. 6. 0. 1. 2. 4. 6. 0. 1. 2. 4. 6. 3.0. -3.5 kb. S’-Y’. S-Y/TSS. 2.0. + IFN g. Relative H4Ac. 1.0. 1. Resistance to MNAse. 0.0. 0.1. 1.2. 0.01. Resistance to MNAse. units:.

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Supplementary figure. 1 Ashino et al.

Supplementary figure. 1 Ashino et al. 6. ■ CCR6. 4. Relative expression of mRNA versus b -actin. 2. 0. Th17 polarized cells. Naive Th cells. Naive Th cells. Th17 polarized cells. DO11.10 Tg. IL-17 -/- DO11.10 Tg. Supplementary Figure 1

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Kudo-Saito, et al Supplementary Figure 1

Kudo-Saito, et al Supplementary Figure 1. Human PBMCs. Debris Dead cells. Leukocytes. Lymphocytes. Kudo-Saito, et al Supplementary Figure 2. Mock D6 D10 F3 F5 NC. Transfectants. Normalization. (a). 0.8 0.6 0.4 0.2 0.0. Snail. Snail Slug Twist E-cadherin Fibronectin

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Voso et al, Supplementary Figure 1

Oligonucleotide sequences and PCR conditions used for sequencing. Voso et al, Supplementary Figure 1. Exemplary sequencing reactions for all genes studied. Voso et al, Supplementary Figure 2. Time to Leukemic evolution. SRSF2-mutated. SRSF2-wild type. Probability (%). p=0.04.

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Supplementary Figure 1 Gattolliat et al

Supplementary Figure 1 Gattolliat et al. Overall survival. Disease -free survival. High miR -487b. High miR -487b. Disease-free surviving (%). Patient surviving (%). Low miR-487b. Low miR-487b. P=0.0671. P=0.0589. Overall survival (months). Disease-Free survival (months).

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Supplementary Figure 1- FUJIMURA et al.

A. Hyp-eIF5A1. Poorly Differentiated. Well Differentiated. 4. Normal. eIF5A1. 4. 3. eIF5A2. 3. C. IHC score. *. *. 2. Poorly Differentiated. Well Differentiated. IHC score. 2. GAPDH. Normal. 1. eIF5A1. eIF5A2. 1. control. 0. normal. Grade II. Grade I. 0.

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