1 / 1

Comparative Analysis of Gene Expression Using Microarray Data and Real-Time PCR

This study compares gene expression values between microarray data and RT-PCR for 7 genes at different developmental stages. The correlation coefficients indicate high concordance. The relative gene expression levels were calculated using the 2-ΔΔCT method.

ranae
Download Presentation

Comparative Analysis of Gene Expression Using Microarray Data and Real-Time PCR

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 6 (A) LEA group 1 (At2g35300.1) 2 -2 -6 -10 r2=0.96 (B) 12S seed storage protein (At1g03880.1) r2=0.94 (C) Ascorbate peroxidase (At1g07890.1) 3 2 1 4 0 (D) ABA-responsive (HVA22b) (At5g62490.1) -1 r2=0.91 0 2 Expression level 0 1 -4 -2 -4 (E) ABA-responsive (At3g02480.1) r2=0.83 -8 6 2 -2 -6 -10 (F) Jacalin lectin family protein (At1g52000.1) 4 2 0 -2 -4 (G) MDHR (At3g52880.1) -6 4 2 0 Stages of development -2 0 1C 2C 3C 1B 2B 3B -4 -6 Appendix 3. Comparisons of expression values for genes selected from the microarray data (open bars) with values generated with real time (RT)-PCR (close bars). The correlation coefficient between the values calculated for each of the 7 genes with microarray and RT-PCR are shown at the bottom of each panel. The relative level of gene expression was analyzed with the 2-∆∆CT method described by Livak and Schmittgen, (2001) using actin (accession number AF111812) as a reference and the common reference (combined pooled of the seven sample stages) as the calibrator r2=0.97 r2=0.93 r2=0.85

More Related