Expression level
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6. (A) LEA group 1 (At2g35300.1). 2. -2. -6. -10. r 2 =0.96. (B) 12S seed storage protein (At1g03880.1). r 2 =0.94. (C) Ascorbate peroxidase ( At1g07890.1). 3. 2. 1. 4. 0. (D) ABA-responsive (HVA22b) (At5g62490.1). -1. r 2 =0.91. 0. 2.

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Expression level

6

(A) LEA group 1 (At2g35300.1)

2

-2

-6

-10

r2=0.96

(B) 12S seed storage protein (At1g03880.1)

r2=0.94

(C) Ascorbate peroxidase

(At1g07890.1)

3

2

1

4

0

(D) ABA-responsive (HVA22b)

(At5g62490.1)

-1

r2=0.91

0

2

Expression level

0

1

-4

-2

-4

(E) ABA-responsive (At3g02480.1)

r2=0.83

-8

6

2

-2

-6

-10

(F) Jacalin lectin family protein

(At1g52000.1)

4

2

0

-2

-4

(G) MDHR (At3g52880.1)

-6

4

2

0

Stages of development

-2

0 1C 2C 3C 1B 2B 3B

-4

-6

Appendix 3. Comparisons of expression values for genes selected from the microarray data (open bars) with values generated with real time (RT)-PCR (close bars).

The correlation coefficient between the values calculated for each of the 7 genes with microarray and RT-PCR are shown at the bottom of each panel. The relative

level of gene expression was analyzed with the 2-∆∆CT method described by Livak and Schmittgen, (2001) using actin (accession number AF111812) as a

reference and the common reference (combined pooled of the seven sample stages) as the calibrator

r2=0.97

r2=0.93

r2=0.85