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Resultados restricción

Resultados restricción. Abril, 2013. Std de peso molecular: Lambda Hind III Ladder. The λ Hind III DNA Ladder is prepared by restriction digestion of phage Lambda DNA to completion with Hind III , followed by heat inactivation of the enzyme .

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Resultados restricción

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  1. Resultados restricción Abril, 2013

  2. Std de peso molecular: Lambda HindIII Ladder • Theλ HindIII DNA Ladderispreparedbyrestrictiondigestion of phage Lambda DNA tocompletionwithHindIII, followedbyheatinactivation of theenzyme. • Theresulting 7 fragmentsrangefrom 564 base pairsto 23.13 kilobases in size. • 564, 2027, 2322, 4361, 6557, 9416, 23130 bp

  3. Gel modelo 1 2 3 4 5 6 T • Std de peso molecular • Sin restringir • Sin muestra • Restringido con 2 enzimas • Restringido con 2 enzimas e incubado toda la noche • Restringido 1 enzima VR VC Inserto T RNA T= topoisómeros; VC=vector de clonación; VR= Vector recombinante (vector+inserto)

  4. Otro gel de Resultado esperado Estándar PR Ez1 Enz2 Topoisomeros Plásmido lineal Inserto Estándar 2 va de 100pb en 100pb hasta llegar el máximo que es de 1000 pb o 1kb

  5. Equipos 1 y 2 P Enz1 Enz2 Std

  6. Equipo 3, 4 y 5 Aquí si se ve inserto, aunque hay otras bandas adicionales

  7. Equipos 6 y 7

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