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CULTURE COLLECTIONS FOR FUTURE: CASE YEASTS FROM GENETIC TO METABOLIC DIVERSITY

CULTURE COLLECTIONS FOR FUTURE: CASE YEASTS FROM GENETIC TO METABOLIC DIVERSITY. Ne ž a Č ade ž and Peter Raspor . Culture collections – link to microbial diversity. 0.004 % area of Earth 0.014 % land of Earth. 1 % of all described species (Mršić , 1997 ).

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CULTURE COLLECTIONS FOR FUTURE: CASE YEASTS FROM GENETIC TO METABOLIC DIVERSITY

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  1. CULTURE COLLECTIONS FOR FUTURE: CASE YEASTS FROM GENETIC TO METABOLIC DIVERSITY Neža Čadež and Peter Raspor

  2. Culturecollections – link to microbialdiversity • 0.004 % areaofEarth • 0.014% landofEarth 1 % ofalldescribedspecies (Mršić, 1997)

  3. The legal base forCulturecollections • The objectivesof the CBD are: • Conservationof biological diversity, • Sustainableuse of its components, • Fair and equitable sharing of the benefits arising from commercial and other utilization of genetic resources. • „Biologicalresourcecentres are anessencial part oftheinfrastructureunderpinninglifesciencesandbiotechnology“.

  4. OECD‘s standardsforculturecollections • Fromculturecollections to BiologicalResourceCentres: • Withaim to providehighqualitybiologicalmaterialsandinformation. • To implementbestpracticesfor BRC as recomendedbyGuidelinespublished in 2007 whichspecify: • Maintenance and calibrationrequirementsfor equipmentcommonlyusedin BRCs • Minimum data sets (MDS) and recommended data sets (RDS) for microbial accessions to BRCs • Quality control procedures recommended for micro-organisms upon receipt • Recommended preservation methods and distribution forms

  5. www.cbs.knaw.nl http://www.dsmz.de

  6. Estimatedcosts to implement OECD standards

  7. Estimated costs to comply with OECD standards

  8. Currenttypesofculturecollections • Publicrepositories • Large, diversecollections • Typestrainsof most (all) species • Reference strains • Patent repository • „Boutiquecollections“ • Narrower range ofspecies • Historicspeciallity: wine, brewing, ecology • Broadeningservices to captureadditionalfunding.

  9. SlovenianCollectionofIndustrialMicroorganisms (ZIM) Preservationmethodsformicroorganisms. http://www.bf.uni-lj.si/zivilstvo/o-oddelku/katedre-in-druge-org-enote/za-biotehnologijo-mikrobiologijo-in-varnost-zivil/zim/

  10. Acquiring new strains from establishmentof ZIM in 1991

  11. Current identification scheme

  12. Researchprojects • Populationdynamicsoffermentedfoods. • Ecologicalfactorsaffectingyeastdiversity. • Yeasts as biocontrolagents. • Diversityofyeastsandtheirimpact on foodquality.

  13. Evaluation of microbial diversity in traditional cheeses from Serbia and Croatia PSALAB No. 195 White-pickledcheese MountainBjelanica, Despotovac, Serbia

  14. Diversityofyeastsand LAB BGLE1 4 0.43 0.16 BGAL3 6 0.45 0.14 BGVL1 4 0.56 0.27 BGBU1 5 0.27 0.02 BGRE2 • 5 • 0.44 • 0.25 ZGPR1 • 4 • 0.46 • 0.43 ZGPR2 • 3 • 0.41 • 0.40 ZGPR3 • 4 • 0.40 • 0.46 ZGBP5 • 3 • 0.29 • 0.58 ZGBP6 • 4 • 0.43 • 0.27 Cheese samples SL H’L DL BGGO5 6 0.7 0.22 BGGO74 0.51 0.34 BGGO11 3 0.32 0.54 BGAL2 3 0.4 0.91 ZGZA7 • 3 • 0.37 • 0.49 ZGBP4 • 5 • 0.47 • 0.39 ZGZA8 • 2 • 0.22 • 1.17 ZGZA9 • 3 • 0.36 • 0.46 Golićetal., IJFM, accepted

  15. Taxonomicstudies - polyphasicapproach to taxonomy www.cbs.knaw.nl www.cbs.knaw.nl

  16. RevisedtaxonomyofHanseniasporaandKloeckera

  17. Hot-spot ofunexploredyeastspeciesdiversity – Slovenian olive oil Yet to bedescribed: Kuraschiasp. nov. andSaturnisporasp. nov.allfromSlovenian olive oil

  18. Futureplans: fromgenetic to metabolicdiversity

  19. Acknowledgement • Aram-Gustav AMALIETTI, Mateja AMBROŽIČ, Teja ARNŠEK (RADEŠČEK), Rosvita ARZENŠEK, Martina AVBELJ (EKERT), Martin BATIČ, Saša BAVEC, Nataša BERGINC, Dušan BERGLEZ, Borut BERNIK, Etijen BIZAJ, Mojca BIZJAK (LAMPE), Marko BLAŽIČ, Iztok BORAK, Vid BORKO, Matjaž BOŠTAR, Sebastjan BREZOVEC, Lučka BRULC, Vesna BUBIĆ, Tatjana BUZETI, Tanja CIGUT, Neža ČADEŽ, Franc ČUŠ, Branka ĐUKIĆ, Alenka FABJANČIČ, Štefan FUJS, Rok GABER, Dušan GORANOVIČ, Aleksandra GORNIK, Romana HABOT, Špela HOEFFERLE, Marinka HORVAT, Stanislava HUDE, Tina IVANČIČ (SEMENIČ), Matilda IVOVIĆ (URUKALO), Polona JAMNIK, Bruna JEJČIČ, Vid JELEN, Špelca JENKO-BRINOVEC, Sergej JERMAN, Simon JESIH, Katja JUG, Tina JURIČ, Borut JUTERŠEK, Barbara KASTELEC, Milena KASTELIC (STANIŠA), Katja KASTELIC (VODOPIVEC), Bojana KESER, Anja KINČIČ, Stanislava KIRINČIČ, Matjaž KIRN, Vida KOCBEK, Nataša KOČAR, Rok KOLIČ, Boris KOVAČ, Nataša KRAČUN, Marija KRIŠTOF (PEČJAK), Mila KRSTIĆ, Darja KRZNAR, Tadeja KUPLENK, Špela LEBAN, Darja LENARČIČ, Ana LENASSI ZUPAN, Urška LEŠNIK, Primož LIKAR, Tina LUKAN, Nina MAVC, Petra , MEDVED, Damjana MIKLIČ MILEK, Tea MORČIČ, Davor OBRADOVIĆ, Klemen OKETIČ, Matej OSET, Andrej OSTERC, Gašper OZIMEK, Maja PAŠ, Katarina PEGAN, Barbara PIŠKUR, Primož PLAHUTA, Sašo PLEVČAK, Aleš PODGORNIK, Janja PODJAVORŠEK, Julijana POLANC, Katja POVHE JEMEC, Sebastjan PRAH, Maja PRINČIČ, Sonja PRPAR, Tomaž RAKUŠA, Jana RAMUŠ, Marjeta RECEK, Nuša RESMAN, Vesna REŽIĆ-DEREANI, Erik RUPNIK, Mihael SIMČIČ, Gordan SLADIČ, Franci SMREKAR, Saša STOJKOVIĆ, Matej STRAŽIŠČAR, Mojca ŠANTAVEC, Dejan ŠARLAH, Kristina ŠAVLI, Maja ŠIKIĆ POGAČAR, Urban ŠILC, Aleš ŠPES, Matija ŠTRLEKAR, Matevž ŠUŠTARIČ, Mirjan ŠVAGELJ, Tilen TRAVNIK, Janja TRČEK, Aljoša TRMČIĆ, Igor TROŠT, Aleksandra USENIK, Ana VELIKONJA, Barbara VOELKNER (KUREŽ), Lilijana VOUK-GRBAC, Tatjana VRŠČAJ VODOŠEK, Tatjana ZAGORC, Tina ZAVAŠNIK-BERGANT, Janez ZAVIRŠEK, Tina ZORMAN, Jure ZUPAN, Igor ŽIVEC, Tadeja ŽOHAR, Andreja ŽUPEC, whofinishedtheirdiplomatheses; • Borut BERNIK, Matjaž BOŠTAR , Sergej JERMAN , Nataša KOČAR , Tatjana KOŠMERL , Boris KOVAČ , Primož PLAHUTA , Aleš PODGORNIK , Artur RACMAN , Jana RAMUŠ , Vesna REŽIĆ-DEREANI , Andreja RIJAVEC BREGAR , Sonja SMOLE MOŽINA , Janja TRČEK , Tatjana ZAGORCwhofinishedtheirmastertheses; • Martin BATIČ, Saša BAVEC, Olga BURICA, Neža ČADEŽ, Irena ČARMAN, Franc ČUŠ, Štefan FUJS, Andrej HOLOBAR, Polona JAMNIK, Špelca JENKO-BRINOVEC, Janja KLINČAR, Miomir KNEŽEVIĆ, Boris KOVAČ, Enej KUŠČER, Ana LENASSI Urška LEŠNIK, Vasilka MAGDEVSKA, Jan MAVRI, Damjana MIKLIČ Andrej OSTERC,Maja PAŠ, Hrvoje PETKOVIĆ, Primož PLAHUTA, Borut POLJŠAK, Katja POVHE Marjeta RECEK, Mihaela SKRT, Sonja SMOLE Franc SMREKAR, Aleš ŠTRANCAR, Janja TRČEK, Vasilij VALENČIČ, Tatjana ZAGORC and Jure ZUPAN who finished their doctoraltheses.

  20. Acknowledgement • prof. dr. Peter Raspor, Head of chair • prof. dr. Sonja SmoleMožina, Vice-Head • doc. dr. NežaČadež • doc. dr. Polona Jamnik • doc. dr. Barbara Jeršek • assist. dr. Maja Paš • dr. Anja Klančnik, Researcher • dr. Marija Kurinčič, Researcher • dr. Jure Zupan, Researcher • Mateja Ambrožič, Researcher • Lidija Baša, Researcher • Katarina Kovač, Researcher • Saša Piskernik, Researcher • Martina Avbelj, Ph D Candidate • Marko Blažič, Ph D Candidate • Janko Kosel, Ph D Candidate • Ana Kramar, Ph D Candidate • Luka Kranjc, Ph D Candidate • Teja Zakrajšek, Ph D Candidate • Matej Šergan, Technical Assistant • DragicaMarković, Secretary

  21. THANK YOU FOR YOUR ATTENTION! HVALA LEPA ZA VAŠO POZORNOST!

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