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PHOTOSYNTHESIS

Explore the process of photosynthesis, its location in plants, and the reactions involved in converting sunlight, water, and carbon dioxide into glucose and oxygen.

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PHOTOSYNTHESIS

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  1. PHOTOSYNTHESIS

  2. SUN photons glucose Photosynthesis Ananabolic, endergonic, carbon dioxide (CO2) requiring process that uses light energy (photons) and water (H2O) to produce organic macromolecules (glucose). 6CO2 + 6H2O  C6H12O6 + 6O2

  3. Question: Where does photosynthesis take place?

  4. Chloroplast Mesophyll Cell Stoma Plants • Autotrophs – produce their own food (glucose) • Process called photosynthesis • Mainly occurs in the leaves: a. stoma - pores b. mesophyll cells

  5. Oxygen (O2) Carbon Dioxide (CO2) Guard Cell Guard Cell Stomata (stoma) Pores in a plant’s cuticle through which water and gases are exchanged between the plant and the atmosphere. Found on the underside of leaves

  6. Nucleus Cell Wall Chloroplast Central Vacuole Mesophyll Cell of Leaf Photosynthesis occurs in these cells!

  7. Stroma Outer Membrane Thylakoid Granum Inner Membrane Chloroplast Organelle where photosynthesistakes place. Thylakoid stacks are connected together

  8. Thylakoid Membrane Thylakoid Space Granum Thylakoid Grana make up the inner membrane

  9. Question: Why are plants green?

  10. Chlorophyll Molecules • Located in the thylakoid membranes • Chlorophyll have Mg+ in the center • Chlorophyll pigments harvest energy (photons) by absorbing certain wavelengths (blue-420 nm and red-660 nm are most important) • Plants are green because the green wavelength is reflected, not absorbed.

  11. 400 500 600 700 Short wave Long wave (more energy) (less energy) Wavelength of Light (nm)

  12. violetbluegreenyelloworangered Absorption of Light by Chlorophyll Absorption wavelength

  13. Question: During the fall, what causes the leaves to change colors?

  14. Fall Colors • In addition to the chlorophyll pigments, there are other pigments present • During the fall, the green chlorophyll pigments are greatly reduced revealing the other pigments • Carotenoids are pigments that are either red, orange, or yellow

  15. Redox Reaction The transfer of one or more electrons from one reactant to another Two types: 1. Oxidation is the loss of e- 2. Reduction is the gain of e-

  16. Oxidation 6CO2 + 6H2O  C6H12O6 + 6O2 glucose Oxidation Reaction The loss of electrons from a substance or the gain of oxygen.

  17. Reduction 6CO2 + 6H2O  C6H12O6 + 6O2 glucose Reduction Reaction The gain of electrons to a substance or the loss of oxygen.

  18. Two Parts of Photosynthesis Two reactions make up photosynthesis: 1.Light Reaction or Light Dependent Reaction - Produces energy from solar power (photons) in the form of ATP and NADPH. SUN

  19. Two Parts of Photosynthesis 2. Calvin Cycle or Light Independent Reaction • Also called Carbon Fixation or C3 Fixation • Uses energy (ATP and NADPH) from light reaction to make sugar (glucose). ATP

  20. Light Reaction (Electron Flow) • Occurs in the Thylakoid membranes (inner membrane) • During the light reaction, there are two possible routes for electron flow A. Cyclic Electron Flow B. Noncyclic Electron Flow

  21. P Cyclic Electron Flow • Occurs in the thylakoid membrane • Uses Photosystem I only • P700 reaction center- chlorophyll a • Uses Electron Transport Chain (ETC) • Generates ATP only ADP + ATP

  22. e- Primary Electron Acceptor SUN e- ATP produced by ETC e- Photons e- P700 Accessory Pigments Photosystem I Cyclic Electron Flow Pigments absorb photons, excite electrons, which produce ATP

  23. Noncyclic Electron Flow • Occurs in the thylakoid membrane • Uses PS II and PS I • P680 reaction center (PSII) - chlorophyll a • P700 reaction center (PS I) - chlorophyll a • Uses Electron Transport Chain (ETC) • Generates O2, ATP and NADPH

  24. Primary Electron Acceptor 2e- Enzyme Reaction Primary Electron Acceptor 2e- 2e- ETC 2e- SUN 2e- NADPH P700 Photon ATP P680 Photon H2O 1/2O2+ 2H+ Photosystem I Photosystem II Noncyclic Electron Flow

  25. P (Reduced) B. Noncyclic Electron Flow • ADP + ATP • NADP+ + H NADPH • Oxygen comes from the splitting of H2O, not CO2 H2O  1/2 O2 + 2H+ (Reduced) (Oxidized)

  26. Chemiosmosis • Powers ATP synthesis. • Located in the thylakoid membranes. • UsesETCand ATP synthase(enzyme) to make ATP. • Photophosphorylation: addition of phosphate to ADP to make ATP.

  27. SUN (Proton Pumping) H+ H+ Thylakoid E T PS I PS II C high H+ concentration H+ H+ H+ H+ H+ H+ Thylakoid Space H+ ATP Synthase low H+ concentration ADP + P ATP H+ Chemiosmosis

  28. Calvin Cycle • Carbon Fixation (light independent rxn). • C3 plants(80% of plants on earth). • Occurs in the stroma. • Uses ATP and NADPH from light rxn. • Uses CO2. • To produce glucose: it takes6 turnsand uses 18 ATP and 12 NADPH.

  29. Stroma Outer Membrane Thylakoid Granum Inner Membrane Chloroplast

  30. (36C) (6C) 6C-C-C-C-C-C (unstable) 6CO2 6C-C-C 6C-C-C 12PGA (36C) 6ATP 6ATP (30C) 6C-C-C-C-C 6NADPH 6NADPH RuBP (36C) 6C-C-C 6C-C-C 6ATP 12G3P (30C) (6C) C3 C-C-C-C-C-C Glucose glucose Calvin Cycle (C3 fixation)

  31. C3 Glucose Calvin Cycle • Remember:C3 = Calvin Cycle

  32. Photorespiration • Occurs on hot, dry, bright days. • Stomates close. • Fixation of O2 instead of CO2. • Produces 2-C molecules instead of 3-C sugar molecules. • Produces no sugar molecules or noATP.

  33. Photorespiration • Because of photorespiration: Plants have special adaptations to limit the effect of photorespiration. 1. C4 plants 2. CAM plants

  34. C4 Plants • Hot, moist environments. • 15% of plants (grasses, corn, sugarcane). • Divides photosynthesis spatially. • Light rxn - mesophyll cells. • Calvin cycle - bundle sheath cells.

  35. Malate Malate C-C-C-C C-C-C-C Transported CO2 CO2 C3 Vascular Tissue glucose C-C-C ATP PEP C-C-C Pyruvic Acid Mesophyll Cell Bundle Sheath Cell C4 Plants

  36. CAM Plants • Hot, dry environments. • 5% of plants (cactus and ice plants). • Stomates closed during day. • Stomates open during the night. • Light rxn - occurs during the day. • Calvin Cycle - occurs when CO2 is present.

  37. Night (Stomates Open) Day (Stomates Closed) Vacuole C-C-C-C C-C-C-C C-C-C-C CO2 Malate Malate Malate CO2 C3 C-C-C ATP C-C-C glucose PEP Pyruvic acid CAM Plants

  38. Question: Why would CAM plants close their stomates during the day?

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