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بسم الله الرحمن الرحيم. Ear Culture. 2013-2014. Diagnostic Medical Microbiology-Laboratory Manual. Ear Culture. Aim of the test Etiological diagnosis of otitis externa or otitis media by aerobic and anaerobic culture with identification and susceptibility test of the isolated organism(s).

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ear culture

بسم الله الرحمن الرحيم

Ear Culture

2013-2014

Diagnostic Medical Microbiology-Laboratory Manual

ear culture1
Ear Culture

Aim of the test

Etiological diagnosis of otitis externa or otitis media by aerobic and anaerobic culture with identification and susceptibility test of the isolated organism(s).

Types of specimen

Two swabs from the external or aspiration from middle ear(s).

Criteria of specimen rejection

Inappropriate specimen transport device; mislabeled specimen; unlabeled specimen; specimen received after prolonged delay (usually more than two hour); specimen received in expired transport media.

pre specimen processing
Pre specimen processing
  • Who will collect the specimen
    • Medical technologist, Microbiologist for swab from external ear.
    • Otolaryngologist for aspiration from middle ear.
  • Who is authorized to order the test
    • Physician.
  • Time relapse before processing the sample
    • Not more than 2 hours.
  • Storage
      • Refrigerated (2-8) 0C.
pre specimen processing1
Pre specimen processing
  • External Ear Specimen collection
    • Collect a specimen of the discharge on a thin, sterile cotton wool or Dacron swab.
    • Place the swab in a container with the transport medium, breaking off the swab stick to allow the stopper to be replaced tightly.
    • Label the specimen and send it to the laboratory.
specimen processing
Specimen processing
  • Ear specimen submitted for culture should be inoculated to blood agar, MacConkey, and chocolate agar plates, anaerobic culture also should be setup on those specimens obtained by tympanocenthesis or those obtained from patients with chronic otitis media, or mastoiditis.
  • Direct visual examination for material aspirated from the middle ear or mastoid is also examined directly for bacteria or fungi.

Cultures from mastoid are generally taken on swabs during surgery and should be transported anaerobically.

additional information
Additional information
  • For external ear infections only Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Vibrio spp. and Aspergillus will be looked for and reported.
  • For middle ear infections only pneumococcus, Streptococcus pyogenes, Haemophilusinfluenzae and Staphylococcus aureuswill be reported with a susceptibility test.
  • For the chronic discharging ear, Bacteroides species and fungi will also be reported in addition to the organisms reported for middle ear infections.
post specimen processing
Post specimen processing
  • Interfering factors:
  • Patient on antibiotic therapy.
  • Improper sample collection.
  • Result reporting:
  • Report Gram stain finding as an initial report.
  • Report the isolated pathogen and its sensitivity pattern as a final report.
  • Turn around time:
  • Gram stain result should be available half hour after specimen receipt.
  • Isolation of a possible pathogen can be expected after 2-4 days.
  • Negative culture will be reported out 1-2 days after the receipt of the specimen.
slide12

END LECTURE

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