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E.HOP: A Bacterial Computer for Pancake Flipping

Explore gene regulation and mathematical modeling in E. coli to solve the Burnt Pancake Problem. Discover practical applications and insights into evolutionary transgenes with unique constructs. Acknowledgments to collaborative institutions.

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E.HOP: A Bacterial Computer for Pancake Flipping

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  1. T T T T T T T T gene regulator (1) 2 + Hin (-1) 2 (1) 2 Single pancake flipping NheI NheI Tet RBS device EX SP -1,2 -2,-1 in 2 flips! T T pSB1A7 rep(pMB1) ampR 1Biology and 2Mathematics Departments, Davidson College, Davidson, North Carolina; 3Biology Department, Hampton University, Hampton, VA E.HOP: A Bacterial Computer to Solve the Pancake ProblemW. Lance Harden2, Sabriya Rosemond3, Samantha Simpson1, Erin Zwack1Mentors: A. Malcolm Campbell1, Karmella Haynes1, and Laurie J. Heyer2 1. The Burnt Pancake Problem 4. Two Pancake Constructs (1,2) (-2,-1) (1,-2) (2,-1) Unsorted Pancakes Problem Solved (-1,2) (-2,1) Can E. coli be programmed to compute solutions? (-1,-2) (2,1) 2. Mathematical Modeling 5. Results *Solution is (1, 2) or (-2, -1) ( 1, 2) (-2, -1) ( 1, -2) (-1, 2) (-2, 1) ( 2, -1) 8 different stacks of two pancakes Assume all possible flips are equally likely Need to build one construct from each “family” (-1, -2) ( 2, 1) Repress pBad-Tet Activate pLac-Hin Screen forOrientation 3. Biological Pancakes 6. Conclusions PRACTICAL Proof-of-concept for bacterial computers Data storage n units gives 2n(n!) combinations BASIC BIOLOGY RESEARCH Improved transgenes in vivo Evolutionary insights Plasmid #1: Two pancakes (Amp vector) Tet RBS hixC hixC hixC pBad Plasmid #2: AraC/Hin generator (Kan vector) AraC PC Hin LVA RBS pSB1K3 pLac Thanks to Missouri Western State University, HHMI, Davidson College, James G. Martin Genomics Program, and MIT

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