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GUS

pGt s. P1. P2. P1: 5’-CATCGTGGACCAAGAAGACGTTG-3’ P2: 5’-ATCGAATTCGATAGAAGTATTTTACAA-3’. pGGGt s. P4. P4. P3. P3. P3: 5’-AGCACTAGTGCTGTCGGCTTTAACCTCT-3’ P4: 5’-ACGTCGACAAAGACTTCGCGCTGATACC-3’. 35S promoter. pG. GUS. P5. P6. 35S terminator.

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GUS

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  1. pGts P1 P2 P1: 5’-CATCGTGGACCAAGAAGACGTTG-3’ P2: 5’-ATCGAATTCGATAGAAGTATTTTACAA-3’ pGGGts P4 P4 P3 P3 P3: 5’-AGCACTAGTGCTGTCGGCTTTAACCTCT-3’ P4: 5’-ACGTCGACAAAGACTTCGCGCTGATACC-3’ 35S promoter pG GUS P5 P6 35S terminator P5: 5’-GTTGGCGGTAACAAGAAAGG-3’ P6: 5’-TTTGCAAGCAGCAGATTACG-3’ nahG T-DNA sequence pN P8 P7 P7: 5’-TCACCTCCCAAAAGGTATCG-3’ P8: 5’-AGAGAGTTGGTGTCGGGATG-3’ Supplemental Figure 3. Locations and sequences of PCR primers for genotyping of F1 progeny from genetic crosses between pGts transformants and the pGGGts, pG or pN transformants.

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