Speaker: 鐘偉銓

1. Metallothionein induction and heavy metal accumulation in white shrimp Litopenaeus vannamei exposed to cadmium and zincJui-Pin Wua, Hon-Cheng Chenb,*Comparative Biochemistry and Physiology, Part C 140 (2005) 383 – 394南美白對蝦暴露於鎘及鋅下之金屬硫蛋白誘發與重金屬蓄積研究 Speaker:鐘偉銓

2. Introduction

4. Materials and methods

6. Control 0.2 mg Cd/l 0.3 mg Zn/l After 3 days Hepatopancreas Centrifuge Sephadex G-75

7. 0 , 0.1 , 0.2mg Cd/l 0, 0.05 , 0.2 mg Zn/l Hepatopancreas(h),gills(g),muscles(m) Polarized Zeeman atomic absorption spectrophotometer

8. Results &Conclusions

9. Normal Cd Zn

11. Cd Zn h g m

13. Discussion

14. The third protein peak is a Zn peak.(Wong and Rainbow,1986) • This indirect method with a direct one revealed that they yielded similar results and trends.(Pederson et al., 1997) • Use MT for monitoring and evaluation the heavy metal pollution was reasonably considered.(Bainy, 2000)

15. Thanks