Cassandra A. Smith. Makah Potato Genomics Workshop June 22-July 2, 2003 USDA/ARS National Science Foundation . Daniel Andrade Dr. Charles Brown Dr. David Culley Neuee V. Ward Tia Beavert Hazel R. Denney Cassandra Smith. Ozette Potato Research Crew.
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
Makah Potato Genomics Workshop
June 22-July 2, 2003
USDA/ARS National Science Foundation
This main reasons we are researching the Ozette potato, because it is already resistant to many diseases and it was found to be rich and antioxidants.
We take notes to better understand the concept of PCR (Polymerase Chain Reaction) and Electroehoresis and what role they play in genetics.
Hazel and I are constructing DNA models to gain a better perspective using a hands-on approach.
Daniel also gives a demonstration on how a double helix is configured.
Certain types of potatoes contain certain amounts of antioxidants.
This is variation in pigmentation from one seed envelope of Solanum phureja from Sturgeon Bay, Wisconsin.
Daniel and Tia are working together to prepare the micro-pipettes to add the buffer.
We begin the extraction process by freezing the potato leaves in microcentrifuge tubes with liquid nitrogen and then grinding them up within the tubes using Konte’s pestles.
Dr. Brown instructs us as we prepare to spin the samples.
Our supervisor Neuee also gets in on the action.
Presented by Hassan Mojtahedi
Dr. Mojahedi carefully explains the wrath of the dreaded nematode and how it can take it’s toll on your crop, especially potatoes.
Dr. Brown give us a review.
Below are infected potato roots.
Figure 3. Development of invading M. chitwoodi in root. A. Solanum tuberosum (susceptible), B. Solanum fendleri. C. Solanum hougasii. D. Solanum bulbocastanum all at 25 days post- inoculation. 60x
Norkotah Russet, Pasco ‘99
PA95A11-14, Pasco ‘99
PA95A11-14, Umatilla ‘99
PA95A11-14, Pasco ‘99
We have to prepare and load gels manually and this can be very time consuming for a beginner.
After rinsing the gel with purified water, acedic acid, and silver nitrate, we begin to see results.
As it turns out the DNA was good, but quite a few things went wrong and we and we had to start over.
Hazel and I are shown separating DNA for size.
Dr. Vandemark explained how a template of DNA could duplicate itself and he also explained how important it is to understand this concept when dealing with disease control.
Over the past several days, our research crew has learned new techniques and acquired enough knowledge to format this power point presentation. We have worked hard in the lab and in our spare time we were on the computers preparing our own personal presentations. And without further ado I present to you a brief collection of us working together on various projects.
Dr. Charles Brown, Kay Brown, Dr. Dave “Tidal wave” Culley, Daniel Andrade, Tia Beavert and Neuee V. Ward.