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Nanopore Membranes for Single-Molecule Detection and Characterization. Anna Schibel, Ryuji Kawano and Henry S. White University of Utah October 26, 2007. The Glass Nanopore (GNP) Membrane . Zhang et al. Anal. Chem. 2007 , 79 , 4778-4787. . Fabrication of the GNP Membrane. Bo Zhang.

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nanopore membranes for single molecule detection and characterization

Nanopore Membranes for Single-Molecule Detection and Characterization

Anna Schibel, Ryuji Kawano and Henry S. White

University of Utah

October 26, 2007

the glass nanopore gnp membrane
The Glass Nanopore (GNP) Membrane

Zhang et al. Anal. Chem. 2007, 79, 4778-4787.

fabrication of the gnp membrane
Fabrication of the GNP Membrane

Bo Zhang

Nanopore membrane

Nanopore electrode

Nanodisk electrode

Zhang, B.; Galusha, J.; Wang, G.; Bergren, A. J.; Jones, R. M.; White, R. J.; Ervin, E. N.; Cauley, C. C.; Shiozawa, P.; White, H. S. Anal. Chem. 2007, 79, 4778-4787.

stochastic single molecule detection
Stochastic Single-Molecule Detection

HL insertion

Single Binding Events

Applied Voltage -40 mV

250 nm pore

1 M KCl

10 mM Potassium

phosphate buffer

pH 7.4

3 M aHL backside

50 M s7CD frontside

Unbound State

Bound State

- -Hemolysin (HL)

Gu et al., Science 2001, 291, 636-640

- hepta-sulfano--cyclodextrin (s7CD)

R = -OSO3-

stochastic single molecule detection5
Stochastic Single-Molecule Detection

i

1 s

a7CD

Electrostatic trapping

Clement Osei Akoto

Rainer Group

R = NH3+

GNP radius - 95 nm

Voltage in wrt cis side

50 M a7CD detection

cis - Reference

Electrostatically trap molecule inside HL channel

trans

+

- a7CD

+

+

+

50 M a7CD

slide6

Nanoparticle Detection

Gold Nanoparticle with Tiopronin Monolayer

2nm

Wang, G., Single Nanoparticle Sensing with Protein Ion Channel Embedded in Lipid Bilayer Supported By Glass Nanopore Membrane (unpublished)

characterization of hairpin dna structures
Characterization of Hairpin DNA Structures

Common structure

9bp-GC

9bp-AT

9bp-TG

discrimination of individual dna hairpin molecules
Discrimination Of Individual DNA Hairpin Molecules

9 bp-TG

9 bp-AT

1 sec

1 sec

1 sec

9 bp-GC

Current

~1 minute

heparin detection
Heparin Detection

-

-

-

-

-

Heparin

applications in development
Applications In Development
  • Biomedical Analysis
    • DNA sequencing
    • Heparin analysis
  • Homeland Defense
    • Chemical warfare agent detection
  • Environmental Analysis
    • Ultra-trace level detection of pollutants
    • Nano-particle analysis