PowerPoint Slideshow about '木賊對 Staphylococcus simulans 之抗菌活性研究' - patrick-leach
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Because people abuse antibiotics, bacterial resistance to antibiotics becomes a serious global problem. Staphylococci are ubiquitous microorganisms important in human and animal infections. Staphylococcus simulans is commonly found in domesticated animals (cattle and swine), that is opportunistic pathogens, in the pathology of epidermitis, otitis and mastitis.Moreover Staphylococcus simulans was identified as the etiological agent of osteomyelitis and septic arthritis in an adult male. In this study, we screened antibacterial activities of 50% ethanolic extracts 87 traditional Chinese medicines to penicillin-resistant Staphylococcus simulans (PRSS), and used disc agar diffusion method, broth dilution method determination of minimum inhibitory concentrations (MICs). The results showed that Equisetum hiemale L. had the best antibacterial activity, and demonstrated the MICs of 384 ug/ml to PRSS. The extract were fractionated into n-Hexane, ethyl acetate, n-Butanol, H2O. The n-Hexane fraction showed MICs of 256 ug/ml to PRSS. We isolated the n-Hexane fraction to get three active compounds against staphylococcus simulans and determined the structure based on the analysis of NMR spectra. We found saringosterol and Aristophyll-C can effectively resist PRSS with MIC of 32 ug/ml and 64 ug/ml individually.Penicillin G 8 ug/ml combined CP2 4 ug/ml and Penicillin G 16 ug/ml combined CP3 16 ug/ml would be to resist PRSS. Conclude the above advantages, the CP2 and CP3 have the potential ability for the development of future antibacterial agent. Key word: staphylococcus simulans; Equisetum hiemale L.; saringosterol; PRSS