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Effects of liquorice on beta- amyloid aggregation and toxicity in Caenorhabditis elegans

Effects of liquorice on beta- amyloid aggregation and toxicity in Caenorhabditis elegans. Pille Link, Prof. Dr. M. Wink University of Heidelberg Institute for Pharmacy and Molecular Biotechnology. Conflict of interest: nothing to declare. Glycyrrhiza glabra. Used to treat ulcers

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Effects of liquorice on beta- amyloid aggregation and toxicity in Caenorhabditis elegans

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  1. Effects of liquorice on beta-amyloid aggregation and toxicity in Caenorhabditiselegans Pille Link, Prof. Dr. M. Wink University of Heidelberg Institute for Pharmacy and Molecular Biotechnology Conflict of interest: nothing to declare

  2. Glycyrrhizaglabra • Used to treat • ulcers • gastritis • cough • longing for sweets

  3. Alzheimer‘sdisease neurofibrillary tangles • oxidative stress • inflammation • neuronal cell death • loss of memory • antioxidant • anti inflammatory • neuroprotective • memory-enhancing http://www.alz.org/braintour/images/ plaques_tangles.jpg G. glabra? Aβ plaques amyloid-β plaques Asl MN & Hosseinzadeh H (2008). Review of Pharmacological Effects of Glycyrrhiza sp. and its Bioactive Compounds. Phytotherapy Research 22: 709-724.

  4. Caenorhabditiselegans: a model for Alzheimer’s disease • CL2006 • Aβ42 expression in musclecells • numberofplaques • CL4176 • temperature inducible Aβ42expression in musclecells • progressive paralysis • Aβ42toxicity Link CD (2006). C. elegansmodels of age-associated neurodegenerative diseases: lessons from transgenic worm models of Alzheimer's disease. Exp Gerontol41: 1007-1013.

  5. Does G. glabrahave an effect on Aβaggregation?

  6. CL2006: thioflavin S staining

  7. Glycyrrhizaglabra isoliquiritigenin (ILG) glycyrrhetinic acid (GRA) glycyrrhizic acid (GA) G. glabra G. glabra concentration: 100 µg/ml * p < 0.01

  8. Can G. glabrareduce Aβ-inducedtoxicity?

  9. Paralysis assay in CL4176 • temperature inducible Aβ expression • paralysis due to Aβ toxicity

  10. Activity against Aβ-toxicity

  11. Conclusions & outlook • extract & pure compounds from G. glabracan: • decrease the number of Aβ plaques • counteract Aβ toxicity • most active compound: isoliquiritigenin • further work: • test more compuonds • clarify the mode of action

  12. Thank you for your attention! IPMB University of Heidelberg

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