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William Kim and Nicole Kiess. The Effect of Fruit Polyphenols on the Longevity of Caenorhabditis elegans. Abstract . The Effect of Fruit Polyphenols on the Longevity of C. elegans.
The Effect of Fruit Polyphenols on the Longevity of C. elegans
Sarcopenia is defined as the loss of muscle mass, strength, and function associated with aging. As aging takes place, there is a sharp decrease in muscle mass and strength seen in both physically active individuals and physically inactive individuals. Sarcopenia is a major feature of aging in both humans and C. elegans, a model organism used for studying aging and longevity. Blueberry polyphenols have been found to increase the lifespan of C. elegans, seen in the pharynx contraction rate in adult C. elegans. C. elegans pumping rate of the pharynx is an indication of the age for the pumping rate of the pharynx decreases with age. Using various concentrations of different fruit polyphenols the lifespan of C. elegans will be measured by observing the pharynx contraction rate. Seventy-five worms will be fed two different concentrations of plum polyphenols, fifty C. elegans will be fed three different concentrations of strawberry polyphenols, and there will be one-hundred fifty worms in the control group. They all will be fed an equal amount of each fruit polyphenol. The average pumping rate of the pharynx per minute will be measured with the use of a Motic camera attached to a Zeiss Axiovert 40 CFL microscope.
Figure 1: Muscle Mass Associated with Aging
Image 1: C. elegans external appearance
Image 2: C. elegans internal appearance
Image 3: C. elegans Life Cycle
Image 4: Pharyngeal Structure of Caenorhabditis elegans
Fig. 2 Blueberry polyphenols extend lifespan and slow aging in C. elegans
Wilson et. al. 2006
Figure 3: Blueberry polyphenols extend lifespan and slow aging in C. elegans
Figure 4: Structural and functional declines in C. elegans pharynx from days 2-10.
Chow et. al. 2006
Figure 5: Visible Structural Decline in C. elegans as a result of Sarcopenia
Herndon et. al. (2002)
Therefore the purpose of this experiment is to test the ability of different concentrations of fruit polyphenols on the prolonging of lifespan in C. elegans.
H(0): The pelargonidin chloride will show no effect on the prolonging of lifespan
in C. elegans.
H(0): The cyanidin chloride will show no effect on the prolonging of lifespan in C. elegans
H(a): The pelargonidin chloride will show a positive increase in the lifespan of C. elegans.
H(a): The cyanidin chloride will show a positive increase in the lifespan of C. elegans.
n = 150
Pelargonidin Chloride 200 ug/ml
n = 50
Pelargonidin Chloride 100ug/ml
n = 50
Pelargonidin Chloride 50ug/ml
n = 50
Cyanidin Chloride 200ug/mg
n = 75
Aging of C. elegans measurement possible with the use of FUDR.
ANOVA run by SPSS followed by a Scheffe Post Hoc Test
N = 150
N = 150
Total Price: $590.95
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