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Caenorhabditis elegans

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Caenorhabditis elegans

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  1. Movie Caenorhabditis elegans The elegant roundworm 1mm

  2. Nematode Parasites Lymphatic filariasis Onchocerciasis

  3. The C. elegans Genome 97 Mb (8x yeast) 19,000 genes (3x yeast) 51% have human homologs

  4. RNAi Nile red staining for fat In C. elegans

  5. The Mechanism of RNAi Movie Animation Overview

  6. ApoptosisProgrammed Cell Death

  7. Hermaphrodites and Males 20um XX XO (1:600) The male has a fancy tail

  8. Fertilization (2n) (2n) Spermatogenesis (n) Oogenesis (n) Lifecycle embryo laying 4 larval instars

  9. Social Interactions Movie Sydney (Brenner) likes worms because they wriggle and you can watch them wriggle -- Frances Crick

  10. Neural Nets and Behavior 302 cells wire the nervous system gamma aminobutyric acid (GABA), acetylcholine (ACh) and dopamine

  11. Gametogenesis syncytium Mitosis Stem cell Meiosis Cellularization

  12. Transgenesis Movie

  13. Mating Movie

  14. Movie Sperm Motility MSP

  15. Development Female Male Pronuclei GFP::histone fusion Movie

  16. Fate Maps

  17. Mitosis Movie

  18. P-granule Partitioning

  19. Setting It All Up Mex-5 Mex-6 Par3/6/aPKC Pie-1 Par-1 Par-2 P-granules

  20. Cell Reorganization Movie Gastrulation occurs as Ea and Ep descend into the embryonic mass

  21. Gastrulation

  22. Making a Worm - Start to Finish Movie

  23. A Few Questions for Thought • Describe the differences between hermaphrodites and males in C. elegans. How are the identities determined and how do they each contribute to the life history of this organism? • Describe the process of oogenesis in a syncytial ovary. • What is RNAi? Apoptosis? Gastrulation? Why are these processes important in this and other organisms? • What is a fate map? How does it apply to the roundworm? • Compare and contrast (in general terms) mosaic and regulatory modes of development • Develop C. elegans as a specific example of mosaic development, explaining how mitotic “daughters” are constrained to different developmental fates based upon inheritance.