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Experiment 3: Microbiology and Experimental Design Bacteria and Fungi on a Kitchen Cutting Board (SEM x2,270) Plastic cutting board surface - rod bacteria in a smooth matrix; filamentous bacteria -smooth oval cells; fungal hyphae (orange) with textured spores

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experiment 3 microbiology and experimental design
Experiment 3: Microbiology and Experimental Design

Bacteria and Fungi on a Kitchen Cutting Board (SEM x2,270)Plastic cutting board surface - rod bacteria in a smooth matrix; filamentous bacteria -smooth oval cells; fungal hyphae (orange) with textured spores

© David Kunkel http://www.denniskunkel.com/

slide2

Objective:

  • to determine whether Dial liquid antibacterial soap kills more Escherichia coli bacteria than SoftSoap non-antibacterial liquid soap
safety
Safety

Scanning electron micrograph of E. coli© David Kunkel

  • The strain of E. coli we are using is not pathogenic.
  • Nevertheless, use reasonable hygienic precautions
  • (hand washing, no food).
  • TAs will discuss sterile technique and equipment.
important points to remember
Important points to remember
  • Do not start centrifuging until the centrifuge is full or no one else will need it in the near future. Use any centrifuge in the lab.
  • Come in tomorrow to count your plates.
  • Do not throw trash in the autoclave bags – they are for items contaminated with bacteria only.
variability and significance
Variability and Significance

Mean height of Lakers team: 2.23 meters

Std. Dev. of Lakers:

0.25 meters

Mean height of Wizards team: 2.28 meters

Std. Dev. of Wizards:

0.22 meters

Wizards mean is 0.05 meters taller, but that difference is much smaller than the variability (SD) in players.

slide6

Mean height of Lakers team: 1.82 meters

Std. Dev. of Lakers:

0.25 meters

Mean height of Wizards team: 2.48 meters

Std. Dev. of Wizards:

0.22 meters

Wizards mean is 0.66 meters taller, and that difference is larger than the variability in players.

next week
Next week

Don’t forget the first test (Sep. 21-22)

  • Format: all multiple choice
  • Review learning objectives
  • Sample questions on Web page