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Blood samples. Kari Kuulasmaa Luxembourg 9-11 April 2008. Uses of blood samples. Lipid measurements Total cholesterol HDL-cholesterol Glucose Fatty acids and lipoproteins Biomarkers E.g. Refl. nutrition (vitamins, flavonoids..) Antibodies Markers of infection and inflammation DNA.

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Blood samples l.jpg

Blood samples

Kari Kuulasmaa

Luxembourg

9-11 April 2008


Uses of blood samples l.jpg
Uses of blood samples

  • Lipid measurements

    • Total cholesterol

    • HDL-cholesterol

  • Glucose

  • Fatty acids and lipoproteins

  • Biomarkers

    • E.g. Refl. nutrition (vitamins, flavonoids..)

  • Antibodies

    • Markers of infection and inflammation

  • DNA


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Blood collection

  • Fasting

    • Not required for cholesterols

    • 8 h (overnight) for glucose

  • By certified phlebotomist

  • Subject in sitting position

  • Minimal use of tourniquet


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Cholesterol

  • Serum preferred

  • Clotting in room temperature for 30-60 min

  • Centrifuging within an hour

  • Analysis preferably on the day of collection

    • or on refrigerated samples within 3 days

    • or frozen samples (max 6 months at -20, longer at -70)


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Glucose

  • Tubes with citrate and fluoride

  • Centrifuge within an hour

  • Analysis preferably on the day of collection

    • or on refrigerated samples within 3 days

    • or frozen samples (max 6 months at -20, longer at -70)


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Change (%) of glucose concentration in whole blood samples

Uchida et al. Clinica Chimica Acta 1988;172:101-108


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Change (%) of glucose concentration in whole blood samples

Citrate-fluoride mix

NaF/K-oxsalate

NaF/EDTA

Li-hep

Time (h)

Väisänen et al. Kliin Lab 2008; 3-4


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Laboratory procedures

  • Use accredited laboratory

  • Good direct enzymatic methods are available

  • CDC has a certification program for clinical diagnostic products for cholesterols

  • External quality control needs to be set up


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