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This study investigates the effect of mutants on Cd tolerance by comparing WT cells with mutants deleted in transporters FET4 and SMF1, and regulator ROX1. Serial dilution assays were conducted to analyze the impact of these mutations on Cd tolerance in yeast cells. For more details, refer to the 'Materials and methods' section.
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Additional data file 5- Broad-range metal uptake system mutants affecting Cd tolerance Serial dilution assays (see ‘Materials and methods’ for details) comparing Cd tolerance of WT cells and of mutant strains deleted in the broad-range transporters FET4 and SMF1, and in the negative FET4 regulator ROX1.