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Mechanotransduction measurements in cells . Modern instrumentation ( phy 651) symposium Department of physics, Syracuse University Eleni Kinfe Degaga. Introduction . Mechanotransduction? How do the cells respond? During differentiation how do the cells decide what they want to be?

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mechanotransduction measurements in cells

Mechanotransduction measurements in cells

Modern instrumentation (phy 651) symposium

Department of physics, Syracuse University

EleniKinfeDegaga

introduction
Introduction
  • Mechanotransduction?
  • How do the cells respond?
  • During differentiation how do the cells decide what they want to be?
  • Structure, dynamics and function
the membrane skeleton of cells
The Membrane Skeleton of Cells

Cell Membrane

Cell

Substrate/Environment

Mechanical Forces between Cell and Substrate

Ankyrin

Spectrin

Actin

P55

4.1

βspectrin

αspectrin

objective
Objective?

- Measuring mechanical stimuli

pressure, Force, Temperature, light intensity, humidity,

And the biological component

Strain, Elasticity, rigidity, stress, tension,Young's Modulus

labview application to mechanotrunsduction
LabVIEW application to mechanotrunsduction
  • For atomization of mechanical stimulus application
  • Measuring the physical characteristics of the stimulus and the biological component
  • Simulation of possible mathematical models relating the mecanotransduction and the cell dynamics
strain measurement simulation
Strain measurement simulation
  • Required materials
  • LabVIEW 2009 containing

- Control Design toolkit 2.1

-Simulation module 8.2

-Strain gauge and half bridge circuit

-Biological organ sample

mathematical model
Mathematical model

V1= [R3/(R3+R4)-R2/(R1+R2)]

V2=[RG+changeinR/2RG-R2/(R1+R2)]Vex

Strain= 2(V2-V1)/VEx(Gf)

pid controller
PID controller
  • Proportional, Integral and differential controlling
  • Proportional (Kp):- reduce rise time & steady state error
  • Integral (Ki):- Eliminates the steady state error, reduces the rise time, inreases settling time
  • Differential (Kd):- Increases the stability, reduce overshoot
references used
References used

*Single Cell Mechanotransduction and Its Modulation Analyzed by Atomic Force Microscope Indentation by Guillaume T. Charras and Mike A. Horton.

*Nuclear shape, mechanics, and mechanotransduction by :-Kris Noel Dahl,1 Alexandre J.S. Ribeiro,1 and Jan Lammerding2.

*Forced unfolding of proteins with in cells by:-Collin P.Johnson, Sin Yao Tang, Christine Carag, David W Speicher,2 Dennis E Discher1,2 Science 317, 663(2007)

* Introduction to LabVIEW for control and design simulation, National Instruments (2009),

*LabVIEW 3D control simulation (2009), Erik Luter,

*Measuring strain with strain guages, National instruments (2008)

*Lessons in electric circuits, Volume 1-DC, Tony R Kuphaldt (2006),