THE MICROSCOPE. Lab 2. Objetives. Be able to define the following terms. a.- coarse adjustment knob i.- field of view b.- fine adjustment knob j.- depth of field c.- stage k.- resolving power
a.- coarse adjustment knob i.- field of view
b.- fine adjustment knob j.- depth of field
c.- stage k.- resolving power
d.- iris diaphragm l.- slide
e.- ocular lens m.- coverslip
f.- objective lens n.- dry mount
g.- parfocal capability o.- wet mount.
1- Ocular lens (eyepiece)Lens through which you view magnified specimen
2- Revolving nosepieceMovable mount for selecting objective lens best suited for
extent magnification desired.
3- Objective lens. Lens on revolving nosepiece which accomplishes the initial
magnification of the specimen.
4- StageFlat work surface upon which the slide is placed. Some microscopes have a mechanical
stage which is used for precise
movement of a slide via control
knobs geared to the stage.
5- Disc diaphragmRegulates the intensity of light passing through the stage aperture and specimen.
7- Stage clips Fit over ends of a slide to hold it in place. A microscope having a mechanical stage has a Lever which is opened laterally (never lifted)To secure the side surfaces of a slide.
8- Coarse adjustment knob Gives gross movement of the nosepiece for initial focus effort.
9- Fine adjustment knob Gives refined movement of the nosepiece for finishing focus.
10- Base Supports the microscope.
At times it can be very useful to know the size of a specimen. This can be accomplished by using the field of view as a scale and simply visualizing it as divisible into ten equal parts. All that remains is to determine the actual length of each 0.1 of the field of view.
c = a x b ___________