All done with mirrors smart substrates for 3d views
Download
1 / 11

All Done with Mirrors: Smart Substrates for 3D Views - PowerPoint PPT Presentation


  • 143 Views
  • Uploaded on

All Done with Mirrors: Smart Substrates for 3D Views. Dr. J. Alexander Liddle Dr. Matthew McMahon, Dr. Andrew Berglund, Dr. Peter Carmichael, Dr. Jabez McClelland. Benefits/Advantages. Microfabricated substrates can be combined with most other optical microscopy techniques Simple

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'All Done with Mirrors: Smart Substrates for 3D Views' - olin


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
All done with mirrors smart substrates for 3d views
All Done with Mirrors: Smart Substrates for 3D Views

Dr. J. Alexander Liddle

Dr. Matthew McMahon, Dr. Andrew Berglund, Dr. Peter Carmichael, Dr. Jabez McClelland


Benefits advantages
Benefits/Advantages

  • Microfabricated substrates can be combined with most other optical microscopy techniques

    • Simple

    • Easy to make

    • Add lots of information

  • Function: Resolve ambiguity

    • Same positions when viewed from above, but different heights

  • Application: Put cell inside sample well

    • Rapid screening of cell height

    • Modest-resolution, high-throughput 3D information


What motivated the research
What Motivated the Research?

Cui, Björk, Liddle, Sönnichsen, Boussert & Alivisatos

Nano Lett.2004, 1093

Solution-Based Nanoparticle Assembly


3d imaging in optical microscope
3D Imaging in Optical Microscope

  • Goal: Monitor/Measure Dynamics by Seeing in 3D

    • Track assembling particles in as close to real-time as possible

      • The smaller they are, the faster they move

  • How?


2d track from 2d images
2D Track from 2D Images

  • 2D tracks easily constructed from 2D video

    • Special software needed to get accurate particle positions

      • Some codes available free


The ideal would be
The ideal would be…

Oh well…hmmm….


Orthogonal tracking microscopy
Orthogonal Tracking Microscopy

Reduce the problem of tracking in 3Dto the problem of tracking in 2D twice

MICROFABRICATED SAMPLE CHAMBER WITH INTEGRATED REFLECTIVE OPTICS

Si


All done with mirrors smart substrates for 3d views

Software Enhances Technology

  • New algorithms developed that are fast and can handle complicated shapes

Difficult

Easy


Demonstration
Demonstration

  • All-silicon substrate

  • Squares defined by photolithography

  • Anisotropic etch of Si by KOH: 54.7° sidewalls

  • Pyramidal indentations

Movie: 190 nm fluorescent polystyrene sphere in water


Future development
Future Development

  • Hardware:

    • Integrate more complex optical elements

      • Better mirrors (i.e. gold)

      • Filters

      • Polarizers

      • Diffractive elements

  • Software

    • Explore asymmetry

      • Asymmetric objects: rotational motion

      • Asymmetric radiation patterns


Summary
Summary

  • Microfabricated substrates can be combined with most other optical microscopy techniques

  • Resolve ambiguity

    • Same positions when viewed from above but different heights

  • Put cell inside sample well

    • Rapid screening of cell height

    • Modest-resolution, high-throughput 3D information

  • A patent application covering the concept of using microfabricated optics to permit imaging in 3D is pending.