1 / 11

Healthbots: Managing Hyperglycemia with E. coli Blood Control

Learn about symptoms, solutions, modifications, and possible outcomes for controlling hyperglycemia using E. coli in the blood. Explore the importance of glucose regulation and potential projects in this innovative approach.

nova
Download Presentation

Healthbots: Managing Hyperglycemia with E. coli Blood Control

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Healthbots:Controlling Hyperglycemia Doug Tischer 5.8.08

  2. Hyperglycemia Acute Symptoms Chronic Symptoms • Excessive hunger • Excessive urination • Excessive thirst • Coma • Blindness • Neuropathy – numbness of toes, feet, fingers • Poor wound healing • Hardening of blood vessels • Kidney disease Normal blood glucose range: 300 – 400 uM http://www.medicinenet.com/diabetes_mellitus/page6.htm http://www.medicinenet.com/diabetes_mellitus/page7.htm#tocm

  3. The Solution • Add E. coli to the blood • Absorb excess glucose if above 400 uM • Chassis: Developed by UC Berekely 2007 iGEM project E. coli http://z.about.com/f/p/440/graphics/images/en/19192.jpg

  4. Abilities Survive for a long time (days) in the blood stream Does not replicate Consume glucose in excess of 400 uM

  5. Glucose Import:Phosphotransferase System (PTS)

  6. Important Genes, Proteins and Functions

  7. Modifications • Decrease EIIBCGlc – Mlc binding affinity • Mlc separates from EIIBCGlc at higher [glucose] • Mlc binds ptsG promoter • Reduced EIIBCGlc expression • Less glucose import • Enhance Mlc inhibition (currently about 18x) • Mutate Mlc DNA binding sites • Add more Mlc binding sites in promoter • Degradation tag on EIIBCGlc • Combats leakiness of (any) inhibitor

  8. Modifications Cont. • ptsG mRNA destabalization • sRNA SgrS binds the mRNA • Promoted by glucose-6-P or analog (α-GM-6-P) • Produce glucose analogs in vivo • Alpha-methyl-glucose

  9. Possible Projects Mutate EIIBCGlc binding site Mutate Mlc (protein) binding site Mutate promoter sequences/add more Mlc binding sites Degradation tag on EIIBCGlc – determine half life

  10. Possible Outcomes Total success: E. coli consume glucose as usual, but stop once extracellular glucose concentrations reach 400 uM. Partial success: E. coli stop consuming glucose at some point, just not at 400 uM. Interesting: Modulated glucose uptake.

  11. References Deutscher, J., Francke, C. and Postma, P.W. 2006. How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bactertia. Microbial and Molecular Biology Reviews. 2006: 939-1031

More Related