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Investigation into Design of Micro Flow Cytometer Data Collection System. Sam Weiers. Nastaran Hashemi , Pouya Asrar. Outline. Motivation and objectives Approach, methods Current Status of work/current results Work in progress, planned work and timeline. Motivation/Objectives.

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investigation into design of micro flow cytometer data collection system

Investigation into Design of Micro Flow Cytometer Data Collection System

Sam Weiers

NastaranHashemi, PouyaAsrar

outline
Outline
  • Motivation and objectives
  • Approach, methods
  • Current Status of work/current results
  • Work in progress, planned work and timeline
motivation objectives
Motivation/Objectives
  • Motivation
    • To create a micro flow cytometer that will analyze marine algae in UUVs
  • Objectives
    • Design and construct an electrical system
      • Run multiple electronic components
      • Collect and save data
approach methods
Approach/Methods
  • First Task: Create a program for data acquisition
  • Tools:
    • LabVIEW 2011
    • NI USB-6351 DAQ Device
    • Excel 2010 (For Graphing)
approach methods2
Approach/Methods
  • Designed and Created Circuits for:
    • 1 PMT
    • 3 MPPCs
approach methods3
Approach/Methods
  • Employ the micro channel into the system
approach methods4
Approach/Methods
  • Employ the micro channel into the system
current status results
Current Status/Results
  • DAQ program operational and stable
  • All MPPC and PMT circuits working
  • Can only collect data from two sources
future work planned
Future Work Planned
  • Add light filters to the system
    • Restrict wavelengths entering PMTs and MPPCs
  • Collect data from all four positions
  • Use living organisms instead of beads
citations
Citations
  • N. Hashemi, J.S. Erickson, J.P. Golden, F.S. Ligler, “Optofluidic Characterization of Marine Algae using a Microflow Cytometer”, Biomicrofluidics, 5, 032009 (2011)
  • PouyaAsrar. Micro Channel Design and Construction.