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Chloroplast Activity Measurement Experiment: DPIP Color Change

Conduct probing experiment exploring chloroplast activity using DPIP color change method. Analyze transmittance at 605 nm under different conditions: Blank, Unboiled Chloroplasts, Boiled Chloroplasts, and Control. Access data spreadsheet.

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Chloroplast Activity Measurement Experiment: DPIP Color Change

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  1. Understanding the Activity of Chloroplasts AP Biology Core Lab # 9

  2. DPIP (Blue) DPIP (Colorless) Measure color change (via transmittance) in spec @ 605 nm

  3. Experimental Conditions • #1) Blank: Chloroplasts + Light -DPIP • #2) Unboiled Chloroplasts – Light + DPIP • #3) Unboiled Chloroplasts + Light + DPIP • #4) Boiled Chloroplasts + Light + DPIP • #5) Control: No Chloroplasts + DPIP WHAT SHOULD HAPPEN TO TRANSMITTANCE IN ALL OF THESE CONDITIONS?

  4. The Data • Click for Excel Spreadsheet

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