Who says science can’t be funny?. Microbial Growth and Metabolism. Chapter 7. Where are they?. Everywhere! (ubiquitous) Not all bacteria can live in every environment Important factors of growth pH Temperature Nutrient/energy sources Gas content Water Salt Radiation.
Elements C,H,O,N,P,S make up 96% of cell
CO2 + H2O (CH2O)n + O2
CO2 + H2S (CH2O)n + Sº + H2O
4H2 + CO2 CH4 + 2H2O
(CH2O)n + O2 CO2 +H2O + energy
Pressure required to prevent the net flow of water across a semipermeable membrane.
Depends on the concentration of dissolved substance in solution and in the cell.
! = Interesting
? = I have a question—write it on the side!
*= important –summarize it
Ex = example
C = connects to something I know
Osmophile = new vocab
- use light scattering method or a Mass Spectrophotometer
- As there is an > in mass (# of cells), there is a < in the amount of light passing through.
- **Counts both living and dead**
- # of colonies present represents the # of living cells in the original suspension
- Use a diluted culture to make counting easier – Serial dilutions. 1ml of culture into each successive solutions.
- **Counts living cells only (dead cells don’t multiply)**
- Difficult with strepto & staphylo forming species
– Count # of bacteria in an accurately measured volume of liquid on a counting chamber/ cytometer slide
- Gridded pattern - **Can’t tell living from dead**