PCR, Viral and Bacterial Genetics. Chps, 18 and 17. Learning Objectives. Describe the process of PCR Explain the use of gel electrophoresis List the essential components of bacterial DNA Compare and contrast transduction, transformation and conjugation as a means of bacterial gene exchange
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Chps, 18 and 17
Produces 8 molecules
Produces 4 molecules
2 molecules produced
to be amplified
Fig. 18-6, p. 378
Well in gel for
Fig. 18-7a, p. 380
Fig. 18-7b, p. 380
An F+ cell conjugates with an F– cell.
One strand of the F factor breaks at a specific point and begins to move from F+ (donor) to F– (recipient) cell as the F factor replicates.
DNA replication of the F factor continues in the donor cell, and a complementary strand to the strand entering the recipient cell begin to be synthesized.
When transfer of the F factor is complete, replication has produced a copy of the F factor in both the donor and recipient cells; the recipient has become an F+. No chromosomal DNA is transferred in this mating.
Fig. 17-4a, p. 356
The F+ cell.
F factor integrates into the E. coli chromosome in a single crossover event.
A cell with integrated F factor—an Hfr donor cell —and an F– cell conjugate. These two cells differ in alleles: the Hfr is a+ b+ c+ d+, and F– cell is a– b– c– d–.
Fig. 17-4b (1), p. 356
Replica plate with
Fig. 17-5a, p. 359
Fig. 17-8, p. 362