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Advanced HILIC-MS Analysis of Arabidopsis thaliana Leaf Extract and Soybean Extract Compounds

This research details the application of HILIC-ESI-MS to analyze polar lipids, anthocyanins, and other compounds from Arabidopsis thaliana leaf extracts. Utilizing a PolyHYDROXYETHYL A capillary column, the study highlights how reversed-phase solute retention differs in HILIC, revealing insights on glucosinolates, amino acids, flavonols, and saccharides. Additionally, it presents methodologies for isocratic analysis of crude soybean extracts, showcasing the efficiency of HILIC-MS in metabolomics research, underlining its capability in yielding high-quality compound profiling.

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Advanced HILIC-MS Analysis of Arabidopsis thaliana Leaf Extract and Soybean Extract Compounds

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  1. H O O H O H C H 3 O O C H O H 3 O H O O O O H O O H O H O H O H C H 3 O H O + O H O O O O O O O H O O O H O C O H O H O O O O H O O O O H O O H O O O O O O H O H O C H O H O H O H O O H O O H C 3 O H O S C S O 3 O H N O H O O H O O H S O H O METABOLOMICS 2006 polar lipids anthocyanins H 3 O H 3 glucosinolates H amino acids flavonols saccharides raffinose sucrose lactose Hex2Glycerol 0 min 90 Arabidopsis thaliana leaf extract HILIC-ESI-MS with a PolyHYDROXYETHYL A capillary 3 µm, 100 Å, 150x0.32 mm Gradient: Decreasing ACN (Courtesy of V.V. Tolstikov, Max Planck Institute-Potsdam)

  2. 33,25 100 90 80 6,96 26,63 70 Relative Abundance 19,63 60 50 29,31 40 30 34,03 74,58 61,39 37,69 20 6,24 85,92 41,22 43,65 77,58 8,26 10 22,53 47,81 17,95 54,04 11,62 72,02 62,62 1,25 85,02 0 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 Time (min) 64,58 100 90 80 70 Relative Abundance 68,42 60 42,39 50 82,23 22,93 40 81,97 61,92 30 22,27 39,47 20 52,12 79,00 39,05 44,71 21,47 83,40 33,95 10 47,64 28,18 2,22 86,27 15,64 3,68 12,64 52,99 72,04 0 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 Time (min) Pipecolic acid 100 50 6.82 0 6.81 100 Lysine Relative Abundance 50 0 8.26 100 Adipicacid 50 0 20.27 100 Saccharopine 50 0 2 4 6 8 10 12 14 16 18 20 22 24 Time (min) THE WORST RETAINED SOLUTES IN REVERSED-PHASE ARE THE BEST-RETAINED IN HILIC! Sample: Arabidopsis thaliana leaf extract HILIC mode: Capillary of PolyHYDROXYETHYL A RPC mode: Capillary of C-18 Hypersil (Courtesy of V. V. Tolstikov, Max Planck Institute-Potsdam) * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * ** 5.70 HILIC-MS/MS: Isocratic analysis of individual compounds from serum or crude extracts in minutes! Sample: Crude soybean extract on PolyHYDROXYETHYL A column (Courtesy of Robert Croes, DuPont Biotech)

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